Phytochemical Analysis and Antibacterial Activity of Tannins Extracted fromSalix albaL. Against Different Gram-Positive and Gram-Negative Bacterial Strains

The present study was aimed to extract tannins from methanolic extracts ofSalix albaand determination of their antimicrobial potential. Antibacterial activity of tannins extracted fromS. albaleaves and stem bark methanolic extracts was determined by using agar well diffusion assay against seven Gram-positive and Gram-negative bacterial strains includingStreptococcus pyogenes, Staphylococcus aureus(1), S. aureus(2), Shigella sonnei, Escherichia coli(1), E. coli(2), andNeisseria gonorrhoeae, and zone of inhibition was measured in terms of millimeter. The highest inhibition zone was recorded at 17.33 +/- 1.15 by using bark tannins (100 mg/ml) againstS. aureus(2) after an incubation period of 48 h while no activity was reported againstS. sonneiby using leaves tannins. It was observed that the tannins from the bark of theS. albawere more effective to kill all bacterial pathogens and the inhibition percentage increased with increasing the concentration of the tannins. Major classes of secondary metabolites were determined qualitatively and quantitatively. Leaves fraction ofS. albamethanolic extract was enriched with Tannins, Quinones, Steroids, Resins, and Phenols while stem bark extracts were embellished with Flavonoids, Tannins, Quinones, and Phenols. Findings from this study will help to develop formulation fromS. albatannins and endorse the ethnopharmacological utilization of the evaluated plant.