Circulating DNA in the blood of gastric cancer patients

被引：62

作者：

Kolesnikova, Elena V. ^{[1
]}

Tamkovich, Svetlana N. ^{[1
,2
]}

Bryzgunova, Olga E. ^{[1
]}

Shelestyuk, Petr I. ^{[3
]}

Permyakova, Valentina I. ^{[4
]}

Vlassov, Valentin V. ^{[1
]}

Tuzikov, Aleksandr S. ^{[5
]}

Laktionov, Pavel P. ^{[1
]}

Rykova, Elena Y. ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Chem Biol & Fundamental Med, Siberian Div, Novosibirsk 630090, Russia

[2] Novosibirsk State Univ, Novosibirsk 630090, Russia

[3] Novosibirsk Oncol Dispensar, Novosibirsk 630108, Russia

[4] Russian Acad Sci, Cent Clin Hosp, Siberian Div, Novosibirsk 630090, Russia

[5] Russian Acad Med Sci, Canc Res Inst, Siberian Div, Tomsk 634009, Russia

来源：

CIRCULATING NUCLEIC ACIDS IN PLASMA AND SERUM V | 2008年 / 1137卷

基金：

俄罗斯基础研究基金会;

关键词：

circulating DNA; cell-surface-bound DNA; hypermethylation; gastric cancer;

D O I：

10.1196/annals.1448.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The concentration of cell-free DNA and promoter methylation status of the MGMT, p15, and hMLH1 genes were analyzed by a fluorescence-based assay and methylation-specific PCR (MSP) in the blood of gastric cancer patients (n = 20) and healthy subjects (n = 22). Gastric cancer patients were characterized by an increased concentration of circulating DNA in the plasma; the amount of cell-surface-bound DNA was not decreased compared with controls and amounted to 80 +/- 15% of the total circulating DNA. MSP analysis of three genes in the cell-surface-bound DNA permits the detection of gastric cancer patients with a sensitivity of 75% and a specificity of 54%. Thus, the cell-surface-bound DNA is a convenient source of DNA for MSP analysis of cancer-specific markers. The data on the presence of methylated DNA in plasma combined with the analysis of other cancer-related changes in DNA can significantly contribute to cancer diagnostics.

引用

页码：226 / 231

页数：6

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