Helicase-mediated changes in RNA structure at the single-molecule level

被引：13

作者：

Koenig, Sebastian L. B. ^{[1
]}

Liyanage, Pramodha S. ^{[2
]}

Sigel, Roland K. O. ^{[1
]}

Rueda, David ^{[2
,3
]}

机构：

[1] Univ Zurich, Inst Inorgan Chem, CH-8057 Zurich, Switzerland

[2] Wayne State Univ, Dept Chem, Detroit, MI 48202 USA

[3] Univ London Imperial Coll Sci Technol & Med, Dept Med, London, England

来源：

RNA BIOLOGY | 2013年 / 10卷 / 01期

基金：

美国国家科学基金会;

关键词：

single-molecule spectroscopy; FRET; PIFE; AFM; optical tweezers; RNA folding; RNA helicase; DEAD-box; DEAD-BOX-PROTEIN; ATOMIC-FORCE MICROSCOPY; VIRUS NS3 HELICASE; RESONANCE ENERGY-TRANSFER; ALTERNATING-LASER EXCITATION; NUCLEIC-ACID TRANSLOCASES; DOUBLE-STRANDED-RNA; II INTRON RIBOZYME; RIG-I; TRANSLATION INITIATION;

D O I：

10.4161/rna.23507

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA helicases are a diverse group of RNA-dependent ATPases known to play a large number of biological roles inside the cell, such as RNA unwinding, remodeling, export and degradation. Understanding how helicases mediate changes in RNA structure is therefore of fundamental interest. The advent of single-molecule spectroscopic techniques has unveiled with unprecedented detail the interplay of RNA helicases with their substrates. In this review, we describe the characterization of helicase-RNA interactions by single-molecule approaches. State-of-the-art techniques are presented, followed by a discussion of recent advancements in this exciting field.

引用

页码：133 / 148

页数：16

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