Cell Cycle-Dependent Phosphorylation of MAN1

被引：14

作者：

Hirano, Yasuhiro ^{[1
]}

Iwase, Yuka ^{[2
]}

Ishii, Kohei ^{[2
]}

Kumeta, Masahiro

Horigome, Tsuneyoshi ^{[2
]}

Takeyasu, Kunio

机构：

[1] Kyoto Univ, Grad Sch Biostudies, Lab Plasma Membrane & Nucl Signaling, Sakyo Ku, Kyoto 6068501, Japan

[2] Niigata Univ, Grad Sch Sci & Technol, Course Fundamental Sci, Nishi Ku, Niigata 9502181, Japan

来源：

BIOCHEMISTRY | 2009年 / 48卷 / 07期

基金：

日本学术振兴会;

关键词：

INNER NUCLEAR-MEMBRANE; LAMIN-B-RECEPTOR; TO-AUTOINTEGRATION FACTOR; XENOPUS EGG EXTRACTS; MITOTIC PHOSPHORYLATION; CAENORHABDITIS-ELEGANS; INTEGRAL PROTEIN; PORE COMPLEX; CDC2; KINASE; LEM DOMAIN;

D O I：

10.1021/bi802060v

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The LEM (LAP2 beta, Emerin, and MAN1) proteins are essential for nuclear membrane targeting to chromatin via an association with barrier-to-autointegration factor (BAF). Herein, we focused on the mitotic phosphorylation of MAN1 and its biological role. MAN1 was phosphorylated in a cell cycle-dependent manner in the Xenopus egg cell-free system, and the mitotic phosphorylation at the N-terminal region of MAN1 suppressed the binding of MAN1 to BAR Titansphere column chromatography followed by MS/MS sequencing identified at least three M-phase-specific phosphorylation sites, Thr-209, Ser-351, and Ser-402, and one cell cycle-independent phosphorylation site, Ser-463. An in vitro BAF binding assay involving mutants S402A and S402E suggested that the phosphorylation of Ser-402 was important for regulation of the binding of MAN1 to BAF.

引用

页码：1636 / 1643

页数：8

共 49 条

[1] Barrier-to-autointegration factor phosphorylation on Ser-4 regulates emerin binding to lamin A in vitro and emerin localization in vivo [J].