Mulinum crassifolium Phil; Two New Mulinanes, Gastroprotective Activity and Metabolomic Analysis by UHPLC-Orbitrap Mass Spectrometry

被引:8
作者
Areche, Carlos [1 ]
Fernandez-Burgos, Ronald [1 ,2 ]
Cano de Terrones, Teresa [2 ]
Simirgiotis, Mario [3 ]
Garcia-Beltran, Olimpo [4 ]
Borquez, Jorge [5 ]
Sepulveda, Beatriz [6 ]
机构
[1] Univ Chile, Fac Ciencias, Dept Quim, Casilla 653, Santiago, Chile
[2] Univ Nacl San Agustin, Dept Quim, Fac Ciencias Nat & Formales, Arequipa 68513, Peru
[3] Univ Austral Chile, Fac Ciencias, Inst Farm, Valdivia 5090000, Chile
[4] Univ Ibague, Fac Ciencias Nat & Matemat, Carrera 22 Calle 67, Ibague 730002, Colombia
[5] Univ Antofagasta, Fac Ciencias Basicas, Dept Quim, Ave Coloso S-N, Antofagasta 1240000, Chile
[6] Univ Andres Bello, Dept Ciencias Quim, Campus Vina Del Mar, Quillota 980, Vina Del Mar, Chile
关键词
diterpenoids; Mulinanes; Mulinum crassifolium; UHPLC-MS; Orbitrap; secondary metabolites; PHENOLIC-COMPOUNDS; AZORELLA-COMPACTA; CHLOROGENIC ACID; RICH EXTRACT; DITERPENOIDS; FRUITS; ISORHAMNETIN; EXPRESSION; FLAVONOIDS; APIGENIN;
D O I
10.3390/molecules24091673
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Mulinum crassifolium Phil. (Apiaceae) is an endemic shrub from Chile commonly used as infusion in traditional medicine to treat diabetes, bronchial and intestinal disorders and stomach ailments, including ulcers. From the EtOAc extract of this plant, the new mulinane-type diterpenoids 3 and 5 were isolated along with three known diterpenoids. The gastroprotective effect of the infusion of the plant was assayed to support the traditional use and a fast HPLC analysis using high resolution techniques was performed to identify the bioactive constituents. The EtOAc extract and the edible infusion showed gastroprotective effect at 100 mg/kg in the HCl/EtOH induced gastric ulcer model in mice, reducing lesions by 33% and 74%, respectively. Finally, a metabolomic profiling based on UHPLC-ESI-MS/HRMS of the edible infusion was performed and thirty-five compounds were tentatively identified including quercetin, caffeic acid, apigenine glucoside, p-coumaric acid, chlorogenic acids, and caffeoylquinic acids, which have been associated previously with gastroprotective and antiulcer properties. This scientific evidence can support the contribution of polyphenols in the gastroprotective activity of the edible infusion of this plant, and can validate at least in part, its ethnopharmacological use.
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页数:12
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