Genetic variations of ND5 gene of mtDNA in populations of Anopheles sinensis (Diptera: Culicidae) malaria vector in China

被引：23

作者：

Makhawi, Abdelrafie M. ^{[1
,2
,3
,4
]}

Liu, Xiao-Bo ^{[1
,2
,3
,5
]}

Yang, Shu-Ran ^{[1
,2
,3
]}

Liu, Qi-Yong ^{[1
,2
,3
,5
]}

机构：

[1] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, Dept Vector Biol & Control, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China

[2] WHO, Collaborating Ctr Vector Surveillance & Managemen, Beijing 102206, Peoples R China

[3] Chinese Ctr Dis Control & Prevent, China CDC Key Lab Surveillance & Early Warning In, Beijing 102206, Peoples R China

[4] Univ Bahri, Coll Appl & Ind Sci, Dept Biotechnol, Khartoum, Sudan

[5] Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China

来源：

PARASITES & VECTORS | 2013年 / 6卷

关键词：

Anopheles sinensis; Population genetics structure; mtDNA; ND5; gene; STATISTICAL TESTS; ENDEMIC REGION; MITOCHONDRIAL; DNA; FLOW; DIFFERENTIATION; ARABIENSIS; THAILAND; GAMBIAE; DIVERGENCE;

D O I：

10.1186/1756-3305-6-290

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Background: Anopheles sinensis is a principal vector for Plasmodium vivax malaria in most parts of China. Understanding of genetic structure and genetic differentiation of the mosquito should contribute to the vector control and malaria elimination in China. Methods: The present study investigated the genetic structure of An. sinensis populations using a 729 bp fragment of mtDNA ND5 among 10 populations collected from seven provinces in China. Results: ND5 was polymorphic by single mutations within three groups of An. sinensis that were collected from 10 different geographic populations in China. Out of 140 specimens collected from 10 representative sites, 84 haplotypes and 71 variable positions were determined. The overall level of genetic differentiation of An. sinensis varied from low to moderate across China and with a F-ST range of 0.00065 - 0.341. Genealogy analysis clustered the populations of An. sinensis into three main clusters. Each cluster shared one main haplotype. Pairwise variations within populations were higher (68.68%) than among populations (31.32%) and with high fixation index (F-ST = 0.313). The results of the present study support population growth and expansion in the An. sinensis populations from China. Three clusters of An. sinensis populations were detected in this study with each displaying different proportion patterns over seven Chinese provinces. No correlation between genetic and geographic distance was detected in overall populations of An. sinensis (R-2 = 0.058; P = 0.301). Conclusions: The results indicate that the ND5 gene of mtDNA is highly polymorphic in An. sinensis and has moderate genetic variability in the populations of this mosquito in China. Demographic and spatial results support evidence of expansion in An. sinensis populations.

引用

页数：11