Plasticity of nuclear and cytoplasmic stress responses of RNA-binding proteins

被引：37

作者：

Backlund, Michael ^{[1
,2
,3
,4
]}

Stein, Frank ^{[2
]}

Rettel, Mandy ^{[2
]}

Schwarzl, Thomas ^{[2
]}

Perez-Perri, Joel, I ^{[2
]}

Brosig, Annika ^{[1
,2
,3
,4
,5
,6
]}

Zhou, Yang ^{[1
,2
,3
,4
]}

Neu-Yilik, Gabriele ^{[1
,2
,3
,4
]}

Hentze, Matthias W. ^{[1
,2
]}

Kulozik, Andreas E. ^{[1
,2
,3
,4
]}

机构：

[1] Heidelberg Univ, Mol Med Partnership Unit MMPU, Neuenheimer Feld 350, D-69120 Heidelberg, Germany

[2] European Mol Biol Lab EMBL, Meyerhofstr 1, D-69117 Heidelberg, Germany

[3] Heidelberg Univ, Dept Pediat Oncol Hematol & Immunol, Neuenheimer Feld 430, D-69120 Heidelberg, Germany

[4] Natl Ctr Tumor Dis NCT, Hopp Childrens Canc Ctr, Neuenheimer Feld 460, D-69120 Heidelberg, Germany

[5] Heidelberg Univ, Collaborat Joint PhD Degree EMBL, D-69120 Heidelberg, Germany

[6] Heidelberg Univ, Fac Biosci, D-69120 Heidelberg, Germany

来源：

NUCLEIC ACIDS RESEARCH | 2020年 / 48卷 / 09期

关键词：

TRANSLATIONAL CONTROL; MASS-SPECTROMETRY; EXPRESSION; IDENTIFICATION; PROTEOME; ENDORIBONUCLEASE; INTERFERENCE; PATHWAYS; PACKAGE; TIA-1;

D O I：

10.1093/nar/gkaa256

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cellular stress causes multifaceted reactions to trigger adaptive responses to environmental cues at all levels of the gene expression pathway. RNA-binding proteins (RBP) are key contributors to stress-induced regulation of RNA fate and function. Here, we uncover the plasticity of the RNA interactome in stressed cells, differentiating between responses in the nucleus and in the cytoplasm. We applied enhanced RNA interactome capture (eRIC) analysis preceded by nucleo-cytoplasmic fractionation following arsenite-induced oxidative stress. The data reveal unexpectedly compartmentalized RNA interactomes and their responses to stress, including differential responses of RBPs in the nucleus versus the cytoplasm, which would have been missed by whole cell analyses.

引用

页码：4725 / 4740

页数：16

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