Isolation and characterization of polymorphic microsatellite markers from Primula nutans (Primulaceae)

被引：2
|
作者：
Kreivi, Marjut [1 ]
Huttunen, Susanna [1 ]
Aspi, Jouni [1 ]
机构：
[1] Oulu Univ, Dept Biol, FIN-90014 Oulu, Finland
来源：
MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 02期
关键词：
microsatellite; Primula nutans; seashore meadow;
D O I：
10.1111/j.1471-8286.2005.01222.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Seven polymorphic microsatellite loci were developed for a perennial seashore plant, Primula nutans. Degenerate oligonucleotide-primed (DOP)-polymerase chain reaction (PCR)-amplified DNA was ligated to TOPO TA vector and screened with radioactively labelled dinucleotide repeat probes. A sample of 378 individuals from Finland, Norway and Russia were used to characterize those loci, which exhibited two to four alleles per locus with observed heterozygosity of 0.003-0.229 and expected heterozygosity of 0.016-0.527. No linkage disequilibrium was found between these seven loci. These are the first microsatellite markers reported for P. nutans.
引用
收藏
页码：334 / 336
页数：3
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