Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection

被引：20

作者：

Alves Barbosa, Rafael Polidoro ^{[1
]}

Galvao Filho, Bruno ^{[1
]}

dos Santos, Luara Isabela ^{[1
]}

Sales Junior, Policarpo Ademar ^{[4
]}

Marques, Pedro Elias ^{[3
]}

Sousa Pereira, Rafaela Vaz ^{[3
]}

Cara, Denise Carmona ^{[3
]}

Bruna-Romero, Oscar ^{[2
]}

Rodrigues, Mauricio Martins ^{[5
,6
]}

Gazzinelli, Ricardo Tostes ^{[1
,4
]}

Machado, Alexandre Vieira ^{[4
]}

机构：

[1] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, Belo Horizonte, MG, Brazil

[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, Belo Horizonte, MG, Brazil

[3] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Morfol, Belo Horizonte, MG, Brazil

[4] Fiocruz MS, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, Brazil

[5] Univ Fed Sao Paulo, Escola Paulista Med, Ctr Terapia Celular & Mol CTCMol, Sao Paulo, Brazil

[6] Univ Massachusetts, Sch Med, Dept Med, Div Infect Dis & Immunol, Worcester, MA USA

来源：

PLOS ONE | 2013年 / 8卷 / 04期

关键词：

CD8(+) T-CELLS; CHAGAS-DISEASE; IMMUNE-RESPONSES; LYMPHOCYTE RESPONSES; BOOST IMMUNIZATION; TRANS-SIALIDASE; VIRUS INFECTION; IFN-GAMMA; A VIRUS; MICE;

D O I：

10.1371/journal.pone.0061795

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In the present study we evaluated the protection raised by immunization with recombinant influenza viruses carrying sequences coding for polypeptides corresponding to medial and carboxi-terminal moieties of Trypanosoma cruzis amastigote surface protein 2 (ASP2). Those viruses were used in sequential immunization with recombinant adenovirus (heterologous prime-boost immunization protocol) encoding the complete sequence of ASP2 (Ad-ASP2) in two mouse strains (C57BL/6 and C3H/He). The CD8 effector response elicited by this protocol was comparable to that observed in mice immunized twice with Ad-ASP2 and more robust than that observed in mice that were immunized once with Ad-ASP2. Whereas a single immunization with Ad-ASP2 sufficed to completely protect C57BL/6 mice, a higher survival rate was observed in C3H/He mice that were primed with recombinant influenza virus and boosted with Ad-ASP2 after being challenged with T. cruzi. Analyzing the phenotype of CD8+ T cells obtained from spleen of vaccinated C3H/He mice we observed that heterologous prime-boost immunization protocol elicited more CD8+ T cells specific for the immunodominant epitope as well as a higher number of CD8+ T cells producing TNF-alpha and IFN-gamma and a higher mobilization of surface marker CD107a. Taken together, our results suggest that immunodominant subpopulations of CD8+ T elicited after immunization could be directly related to degree of protection achieved by different immunization protocols using different viral vectors. Overall, these results demonstrated the usefulness of recombinant influenza viruses in immunization protocols against Chagas Disease.

引用

页数：11

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