Light-induced cell detachment for cell sheet technology

被引：103

作者：

Hong, Yi ^{[1
]}

Yu, Mengfei ^{[2
]}

Weng, Wenjian ^{[1
,3
]}

Cheng, Kui ^{[1
]}

Wang, Huiming ^{[2
]}

Lin, Jun ^{[2
]}

机构：

[1] Zhejiang Univ, Dept Mat Sci & Engn, State Key Lab Silicon Mat, Hangzhou 310027, Zhejiang, Peoples R China

[2] Zhejiang Univ, Affiliated Hosp 1, Coll Med, Hangzhou 310003, Zhejiang, Peoples R China

[3] Chinese Acad Sci, Shanghai Inst Ceram, Shanghai 200050, Peoples R China

来源：

BIOMATERIALS | 2013年 / 34卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Cell detachment; Light-induced; Titanium dioxide; Nanodots film; ENDOTHELIAL-CELLS; DNA-DAMAGE; ADHESION; GROWTH; SURFACES; CARCINOGENESIS; OXIDATION;

D O I：

10.1016/j.biomaterials.2012.09.043

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The phenomenon of light-induced cell detachment is reported. Mouse calvaria-derived, pre-osteoblastic (MC3T3-E1) cells were cultured on a TiO2 nanodot-coated quartz substrate. After 20 min of UV365 illumination, over 90% of the cells would detach from the surface. Moreover, intact cell sheets could be obtained in the same way. It was found that the as-obtained cells showed good viability, and could be used for further culture processes and other applications. Also, biocompatibility and safety characterizations indicated that the use of TiO2 nanodots and UV365 illumination was safe for such cell detachment. It is suggested that adsorbed extracellular matrix proteins play key roles in developing cell sheets and ensuring biocompatibility. The present light-induced cell detachment method demonstrates a promising way for rapid cell/cell sheet harvesting. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：11 / 18

页数：8

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