Proteoform Suite: Software for Constructing, Quantifying, and Visualizing Proteoform Families

被引：31

作者：

Cesnik, Anthony J. ^{[1
]}

Shortreed, Michael R. ^{[1
]}

Schaffer, Leah V. ^{[1
]}

Knoener, Rachel A. ^{[1
]}

Frey, Brian L. ^{[1
]}

Scalf, Mark ^{[1
]}

Solntsev, Stefan K. ^{[1
]}

Dai, Yunxiang ^{[1
]}

Gasch, Audrey P. ^{[2
,3
]}

Smith, Lloyd M. ^{[1
,3
]}

机构：

[1] Univ Wisconsin, Dept Chem, 1101 Univ Ave, Madison, WI 53706 USA

[2] Univ Wisconsin, Genet Lab, Madison, WI 53706 USA

[3] Univ Wisconsin, Genome Ctr Wisconsin, Madison, WI 53706 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2018年 / 17卷 / 01期

关键词：

proteoform; identification; quantification; visualization; modification; PTM; NeuCode; intact mass; lysine count; TOP-DOWN PROTEOMICS; ENCODED MASS SIGNATURES; INTACT-MASS; PROTEIN; DISCOVERY; YEAST; PHOSPHORYLATION; ELECTROPHORESIS; SPECTROMETRY; TRANSLATION;

D O I：

10.1021/acs.jproteome.7b00685

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present an open-source, interactive program named Proteoform Suite that uses proteoform mass and intensity measurements from complex biological samples to identify and quantify proteoforms. It constructs families of proteoforms derived from the same gene, assesses proteoform function using gene ontology (GO) analysis, and enables visualization of quantified proteoform families and their changes. It is applied here to reveal systemic proteoform variations in the yeast response to salt stress.

引用

页码：568 / 578

页数：11

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