Evidence that the genomic RNA of hepatitis E virus is capped

被引：101

作者：

Kabrane-Lazizi, Y ^{[1
]}

Meng, XJ

Purcell, RH

Emerson, SU

机构：

[1] NIAID, Infect Dis Lab, Hepatitis Viruses Sect, NIH, Bethesda, MD 20892 USA

[2] NIAID, Infect Dis Lab, Mol Hepatitis Sect, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF VIROLOGY | 1999年 / 73卷 / 10期

关键词：

D O I：

10.1128/JVI.73.10.8848-8850.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis E virus (HEV) is an unclassified virus with a positive-sense RNA genome and an undefined replication strategy. In order to determine whether the HEV genome is capped or not, we developed a reverse transcription-PCR assay that is based on the ability of a monoclonal antibody to recognize 7-methylguanosine (m(7)G), Antibody to m7G bound RNA extracted from virions of two different HEV genotypes, The cap analog competitively inhibited the binding of virion RNAs, demonstrating that HEV has a capped RNA genome.

引用

页码：8848 / 8850

页数：3

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