Evaluation of the specificity of four commercially available antibodies to alpha-syntrophin

被引：6

作者：

Eisinger, Kristina ^{[1
]}

Froehner, Stanley C. ^{[2
]}

Adams, Marvin E. ^{[2
]}

Krautbauer, Sabrina ^{[1
]}

Buechler, Christa ^{[1
]}

机构：

[1] Univ Hosp Regensburg, Dept Internal Med 1, D-93042 Regensburg, Germany

[2] Univ Washington, Dept Physiol & Biophys, Seattle, WA 98195 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2015年 / 484卷

关键词：

Immunoblot; Knockout mice; Liver; LOCALIZATION; PROTEINS;

D O I：

10.1016/j.ab.2015.06.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Alpha-syntrophin (SNTA) is an adaptor protein that regulates several signaling pathways. To analyze expression of SNTA immunoblot assays must be performed. Here, the specificity of four commercially available SNTA antibodies has been evaluated in immunoblot experiments using liver tissues of wild-type and SNTA-deficient mice. While one of the antibodies reacts with SNTA, two antibodies specifically recognize beta 2 syntrophin (SNTB2). The antigen detected by the fourth antibody has not been identified but is different from SNTA and SNTB2. Therefore, only one of the four tested antibodies is appropriate to analyze SNTA protein levels by immunoblot. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：99 / 101

页数：3

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