Analysis of HIV type 1 subtype C full-length gag gene sequences from India:: Novel observations and plausible implications

被引：17

作者：

Gupta, RM

Prasad, VVSP

Rai, A

Seth, P ^{[1
]}

机构：

[1] Armed Forces Med Coll, Dept Microbiol, Pune, Maharashtra, India

[2] All India Inst Med Sci, Dept Microbiol, New Delhi 110029, India

[3] Natl Inst Communicable Dis, Dept Biotechnol, New Delhi, India

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 2005年 / 21卷 / 12期

关键词：

D O I：

10.1089/aid.2005.21.1066

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Twenty-four HIV-1 gag genes from patients in India were sequenced and analyzed. All measured 1476-1491 nucleotides with an average of 1483 nucleotides in length. Phylogenetic analysis revealed a homogeneous epidemic of HIV-1 subtype C. Intragenotypic divergence of up to 6.6% was present. Fourteen novel conserved signature pattern residues were delineated for HIV-1 subtype C strains. Each of the 15 nucleocapsid (NC) basic residues was highly conserved p6 gag LXSLFG and PT/SAPP motifs were highly conserved except for PTVPT in three and PTAPT in two strains. Zinc ringer motifs were conserved in all. Documented HIV-1 subtype C gag immunodominant CTL epitopes were conserved. The evolving predominance and the change in nature of the epidemic from Thai B to that of subtype C in the Northeastern regions of India were observed. Tracking the evolution of the Indian epidemic has implications for developing a vaccine.

引用

页码：1066 / 1072

页数：7

共 20 条

[1]

*AIDS EP UPD, 2004, UNNAIDS

[2]

[Anonymous], 1997, EMBnet News

[3] Human immunodeficiency virus type 1 virion density is not determined by nucleocapsid basic residues [J].

Cimarelli, A ;

Luban, J .

JOURNAL OF VIROLOGY, 2000, 74 (15) :6734-6740

[4] AIDS - Diversity considerations in HIV-1 vaccine selection [J].

Gaschen, B ;

Taylor, J ;

Yusim, K ;

Foley, B ;

Gao, F ;

Lang, D ;

Novitsky, V ;

Haynes, B ;

Hahn, BH ;

Bhattacharya, T ;

Korber, B .

SCIENCE, 2002, 296 (5577) :2354-2360

[5] EFFECT OF MUTATIONS AFFECTING THE P6 GAG PROTEIN ON HUMAN-IMMUNODEFICIENCY-VIRUS PARTICLE RELEASE [J].

GOTTLINGER, HG ;

DORFMAN, T ;

SODROSKI, JG ;

HASELTINE, WA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (08) :3195-3199

[6] Differential narrow focusing of immunodominant human immunodeficiency virus Gag-specific cytotoxic T-lymphocyte responses in infected African and Caucasoid adults and children [J].

Goulder, PJR ;

Brander, C ;

Annamalai, K ;

Mngqundaniso, N ;

Govender, U ;

Tang, Y ;

He, S ;

Hartman, KE ;

O'Callaghan, CA ;

Ogg, GS ;

Altfeld, MA ;

Rosenberg, ES ;

Cao, H ;

Kalams, SA ;

Hammond, M ;

Bunce, M ;

Pelton, SI ;

Burchett, SA ;

McIntosh, K ;

Coovadia, HM ;

Walker, BD .

JOURNAL OF VIROLOGY, 2000, 74 (12) :5679-5690

[7]

Hall T.A., 1999, NUCL ACIDS S SER, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008

[8]

Kumar S., 2001, MEGA MOL EVOLUTIONAR

[9] Genetic and serologic characterization of HIV type 1 prevailing in Myanmar (Burma) [J].

Kusagawa, S ;

Sato, H ;

Watanabe, S ;

Nohtomi, K ;

Kato, K ;

Shiino, T ;

Thwe, M ;

Oo, KY ;

Lwin, S ;

Mra, R ;

Kywe, B ;

Yamazaki, S ;

Takebe, Y .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 1998, 14 (15) :1379-1385

[10] PHYLOGENETIC ANALYSIS OF GAG GENES FROM 70 INTERNATIONAL HIV-1 ISOLATES PROVIDES EVIDENCE FOR MULTIPLE GENOTYPES [J].

LOUWAGIE, J ;

MCCUTCHAN, FE ;

PEETERS, M ;

BRENNAN, TP ;

SANDERSBUELL, E ;

EDDY, GA ;

VANDERGROEN, G ;

FRANSEN, K ;

GERSHYDAMET, GM ;

DELEYS, R ;

BURKE, DS .

AIDS, 1993, 7 (06) :769-780

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