Preparation of liposomes from milk fat globule membrane phospholipids using a Microfluidizer

被引:100
作者
Thompson, AK [1 ]
Singh, H [1 ]
机构
[1] Massey Univ, Riddet Ctr, Palmerston North, New Zealand
关键词
milk fat globule membrane; liposome; encapsulation; Microfluidizer;
D O I
10.3168/jds.S0022-0302(06)72105-1
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The isolation of milk fat globule membrane (MFGM) material from buttermilk on a commercial scale has provided a new ingredient rich in phospholipids and sphingolipids. An MFGM-derived phospholipid fraction was used to produce liposomes via a high-pressure homogenizer (Microfluidizer). This technique does not require the use of solvents or detergents, and is suitable for use in the food industry. The liposome dispersion had an average hydrodynamic diameter of 95 nm, with a broad particle-size distribution. Increasing the number of passes through the Microfluidizer, increasing the pressure, or reducing the phospholipid concentration all resulted in a smaller average liposome diameter. Changing these variables did not have a significant effect on the polydispersity of the dispersion. Electron microscopy showed that the dispersions formed had a range of structures, including unilamellar, multilamellar, and multivesicular liposomes. The composition of the MFGM phospholipid material is different from that of the phospholipids usually used for liposome production in the pharmaceutical and cosmetic industries. The MFGM-derived fraction comprises approximately 25% sphingomyelin, and the fatty acids are primarily saturated and monounsaturated. These differences are likely to affect the properties of the liposomes produced from the phospholipid material, and it may be possible to exploit the unique composition of the MFGM phospholipid fraction in the delivery of bioactive ingredients in functional foods.
引用
收藏
页码:410 / 419
页数:10
相关论文
共 42 条
  • [1] Invisible liposomes:: Refractive index matching with sucrose enables flow dichroism assessment of peptide orientation in lipid vesicle membrane
    Ardhammar, M
    Lincoln, P
    Nordén, B
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (24) : 15313 - 15317
  • [2] Concentration of polar MFGM lipids from buttermilk by microfiltration and supercritical fluid extraction
    Astaire, JC
    Ward, R
    German, JB
    Jiménez-Flores, R
    [J]. JOURNAL OF DAIRY SCIENCE, 2003, 86 (07) : 2297 - 2307
  • [3] PREPARATION OF LIPOSOMES USING A MINI-LAB-8.30-H HIGH-PRESSURE HOMOGENIZER
    BACHMANN, D
    BRANDL, M
    GREGORIADIS, G
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1993, 91 (01) : 69 - 74
  • [4] Factors involved in the production of liposomes with a high-pressure homogenizer
    Barnadas-Rodríguez, R
    Sabés, M
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2001, 213 (1-2) : 175 - 186
  • [5] BETAGERI GV, 1999, MICROSPHERES MICROCA, V1, P489
  • [6] Monomolecular collapse of plasmid DNA into stable virus-like particles
    Blessing, T
    Remy, JS
    Behr, JP
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (04) : 1427 - 1431
  • [7] Isolation and characterization of whey phospholipids
    Boyd, LC
    Drye, NC
    Hansen, AP
    [J]. JOURNAL OF DAIRY SCIENCE, 1999, 82 (12) : 2550 - 2557
  • [8] Preparation and characterization of semi-solid phospholipid dispersions and dilutions thereof
    Brandl, M
    Drechsler, M
    Bachmann, D
    Tardi, C
    Schmidtgen, M
    Bauer, KH
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1998, 170 (02) : 187 - 199
  • [9] Vesicle size, size distribution, stability, and rheological properties of liposomes coated with water-soluble chitosans of different molecular weights and concentrations
    Chen, RH
    Win, HP
    Fang, HJ
    [J]. JOURNAL OF LIPOSOME RESEARCH, 2001, 11 (2-3) : 211 - 228
  • [10] Production of a novel ingredient from buttermilk
    Corredig, M
    Roesch, RR
    Dalgleish, DG
    [J]. JOURNAL OF DAIRY SCIENCE, 2003, 86 (09) : 2744 - 2750