Simultaneous saccharification and fermentation of paper sludge without pretreatment using cellulase from Acremonium cellulolyticus and thermotolerant Saccharomyces cerevisiae

被引：18

作者：

Dwiarti, Lies ^{[4
]}

Boonchird, Chuenchit ^{[3
]}

Harashima, Satoshi ^{[2
]}

Park, Enoch Y. ^{[1
,4
]}

机构：

[1] Shizuoka Univ, Grad Sch Sci & Technol, Biotechnol Lab, Integrated Biosci Sect,Suruga Ku, Shizuoka 4228017, Japan

[2] Mahidol Univ, Fac Sci, Dept Biotechnol, Bangkok 10400, Thailand

[3] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Osaka, Japan

[4] Shizuoka Univ, Fac Agr, Biotechnol Lab, Shizuoka 4228017, Japan

来源：

BIOMASS & BIOENERGY | 2012年 / 42卷

基金：

日本科学技术振兴机构;

关键词：

Acremonium cellulolyticus; Biorefinery; Thermotolerant Saccharomyces cerevisiae; Cellulase; Simultaneous saccharification and fermentation; ETHANOL-PRODUCTION; CO-FERMENTATION; BETA-GLUCOSIDASE; HIGH-TEMPERATURE; FUEL ETHANOL; CONVERSION; CULTURE;

D O I：

10.1016/j.biombioe.2012.02.019

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The treatment and reutilization of paper sludge (PS) is a worldwide issue. In this study, PS without any pretreatment was used for bioethanol production through simultaneous saccharification and fermentation (SSF) using hyper producer of cellulase and thermotolerant yeast. For saccharification of PS cellulose, cellulase activity was improved from 1.28 x 10(7) FPU m(-3) (wild type) up to 1.52 x 10(7) FPU m(-3) by mutation of Acremonium cellulolyticus C-1 and its carboxymethyl cellulase (CMCase) activity increased 5-fold compared to that of the wild type. In the SSF process, using A. cellulolyticus origin cellulase the highest level of ethanol production was 11.80 and 11.34 kg m(-3) from 50 kg m(-3) untreated PS in 250 cm(3) and 2 dm(3) flask scales, respectively. Ethanol yield based on consumed glucose was 80%. This result exhibits a high ethanol production yield from untreated PS, without using a commercial cellulase, nor any pretreatment of PS during SSF process. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：114 / 122

页数：9

共 35 条

[1] High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast? [J].

Abdel-Banat, Babiker M. A. ;

Hoshida, Hisashi ;

Ano, Akihiko ;

Nonklang, Sanom ;

Akada, Rinji .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2010, 85 (04) :861-867

[2] Combustion characteristics of biomass in SouthEast Asia [J].

Aghamohammadi, Nasrin ;

Sulaiman, Nik Meriam Nik ;

Aroua, Mohamed Kheireddine .

BIOMASS & BIOENERGY, 2011, 35 (09) :3884-3890

[3]

BARTON RG, 1991, PROCESS SAF ENVIRON, V69, P29

[4]

Brachmann CB, 1998, YEAST, V14, P115

[5]

Cai YJ, 1999, APPL ENVIRON MICROB, V65, P553

[6] Conversion of paper sludge to ethanol. I: Impact of feeding frequency and mixing energy characterization [J].

Fan, Zhiliang ;

Lynd, Lee R. .

BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2007, 30 (01) :27-34

[7] Strain improvement of Acremonium cellulolyticus for cellulase production by mutation [J].

Fang, Xu ;

Yano, Shinichi ;

Inoue, Hiroyuki ;

Sawayama, Shigeki .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2009, 107 (03) :256-261

[8] Enzymatic hydrolyzing performance of Acremonium cellulolyticus and Trichoderma reesei against three lignocellulosic materials [J].

Fujii, Tatsuya ;

Fang, Xu ;

Inoue, Hiroyuki ;

Murakami, Katsuji ;

Sawayama, Shigeki .

BIOTECHNOLOGY FOR BIOFUELS, 2009, 2

[9] MEASUREMENT OF CELLULASE ACTIVITIES [J].

GHOSE, TK .

PURE AND APPLIED CHEMISTRY, 1987, 59 (02) :257-268

[10] Preparation of sodium carboxymethyl cellulose from paper sludge [J].

He, Xiaojia ;

Wu, Shaozu ;

Fu, Dongkang ;

Ni, Jinren .

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2009, 84 (03) :427-434

← 1 2 3 4 →