Complete genome sequence of Bacillus velezensis M75, a biocontrol agent against fungal plant pathogens, isolated from cotton waste

被引：48

作者：

Kim, Sang Yoon ^{[1
]}

Lee, Sang Yeob ^{[1
]}

Weon, Hang-Yeon ^{[1
]}

Sang, Mee Kyung ^{[1
]}

Song, Jaekyeong ^{[1
]}

机构：

[1] RDA, Natl Inst Agr Sci NAS, Agr Microbiol Div, Seoul 55365, South Korea

来源：

JOURNAL OF BIOTECHNOLOGY | 2017年 / 241卷

关键词：

Antifungal activity; Bacillus velezensis M75; Complete genome sequencing; Fungal plant pathogens; Secondary metabolites; FUNCTIONAL-CHARACTERIZATION; GENE CLUSTERS; FERMENTATION; MERSACIDIN;

D O I：

10.1016/j.jbiotec.2016.11.023

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacillus species have been widely used as biological control agents in agricultural fields due to their ability to suppress plant pathogens. Bacillus velezensis M75 was isolated from cotton waste used for mushroom cultivation in Korea, and was found to be antagonistic to fungal plant pathogens. Here, we report the complete genome sequence of the M75 strain, which has a 4,007,450-bp single circular chromosome with 3921 genes and a G+C content of 46.60%. The genome contained operons encoding various non ribosomal peptide synthetases and polyketide synthases, which are responsible for the biosynthesis of secondary metabolites. Our results will provide a better understanding of the genome of B. velezensis strains for their application as biocontrol agents against fungal plant pathogens in agricultural fields. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：112 / 115

页数：4

共 20 条

[1]

Borriss R, 2013, MOL MICROBIAL ECOLOG, P883

[2]

Borriss R, 2011, BACTERIA IN AGROBIOLOGY: PLANT GROWTH RESPONSES, P41, DOI 10.1007/978-3-642-20332-9_3

[3] MERSACIDIN, A NEW ANTIBIOTIC FROM BACILLUS FERMENTATION, ISOLATION, PURIFICATION AND CHEMICAL CHARACTERIZATION [J].

CHATTERJEE, S ;

LAD, SJ ;

PHANSALKAR, MS ;

RUPP, RH ;

GANGULI, BN ;

FEHLHABER, HW ;

KOGLER, H .

JOURNAL OF ANTIBIOTICS, 1992, 45 (06) :832-838

[4] Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens [J].

Chen, X. H. ;

Koumoutsi, A. ;

Scholz, R. ;

Schneider, K. ;

Vater, J. ;

Suessmuth, R. ;

Piel, J. ;

Borriss, R. .

JOURNAL OF BIOTECHNOLOGY, 2009, 140 (1-2) :27-37

[5] Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens are efficient in controlling fire blight disease [J].

Chen, X. H. ;

Scholz, R. ;

Borriss, M. ;

Junge, H. ;

Moegel, G. ;

Kunz, S. ;

Borriss, R. .

JOURNAL OF BIOTECHNOLOGY, 2009, 140 (1-2) :38-44

[6] Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42 [J].

Chen, Xiao Hua ;

Koumoutsi, Alexandra ;

Scholz, Romy ;

Eisenreich, Andreas ;

Schneider, Kathrin ;

Heinemeyer, Isabelle ;

Morgenstern, Burkhard ;

Voss, Bjoern ;

Hess, Wolfgang R. ;

Reva, Oleg ;

Junge, Helmut ;

Voigt, Birgit ;

Jungblut, Peter R. ;

Vater, Joachim ;

Suessmuth, Roderich ;

Liesegang, Heiko ;

Strittmatter, Axel ;

Gottschalk, Gerhard ;

Borriss, Rainer .

NATURE BIOTECHNOLOGY, 2007, 25 (09) :1007-1014

[7] Structural and functional characterization of three polyketide synthase gene clusters in Bacillus amyloliquefaciens FZB 42 [J].

Chen, Xiao-Hua ;

Vater, Joachim ;

Piel, Joern ;

Franke, Peter ;

Scholz, Romy ;

Schneider, Kathrin ;

Koumoutsi, Alexandra ;

Hitzeroth, Gabriele ;

Grammel, Nicolas ;

Strittmatter, Axel W. ;

Gottschalk, Gerhard ;

Suessmuth, Roderich D. ;

Borriss, Rainer .

JOURNAL OF BACTERIOLOGY, 2006, 188 (11) :4024-4036

[8] Bacillus velezensis is not a later heterotypic synonym of Bacillus amyloliquefaciens; Bacillus methylotrophicus, Bacillus amyloliquefaciens subsp plantarum and 'Bacillus oryzicola' are later heterotypic synonyms of Bacillus velezensis based on phylogenomics [J].

Dunlap, Christopher A. ;

Kim, Soo-Jin ;

Kwon, Soon-Wo ;

Rooney, Alejandro P. .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2016, 66 :1212-1217

[9] The Genome of Plant Growth-Promoting Bacillus amyloliquefaciens subsp plantarum Strain YAU B9601-Y2 Contains a Gene Cluster for Mersacidin Synthesis [J].

Hao, Kun ;

He, Pengfei ;

Blom, Jochen ;

Rueckert, Christian ;

Mao, Zichao ;

Wu, Yixin ;

He, Yueqiu ;

Borriss, Rainer .

JOURNAL OF BACTERIOLOGY, 2012, 194 (12) :3264-3265

[10] Structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in Bacillus amyloliquefaciens strain FZB42 [J].

Koumoutsi, A ;

Chen, XH ;

Henne, A ;

Liesegang, H ;

Hitzeroth, G ;

Franke, P ;

Vater, J ;

Borriss, R .

JOURNAL OF BACTERIOLOGY, 2004, 186 (04) :1084-1096

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