Staphylococcus aureus interaction with Pseudomonas aeruginosa biofilm enhances tobramycin resistance

被引:122
作者
Beaudoin, T. [1 ]
Yau, Y. C. W. [2 ]
Stapleton, P. J. [2 ]
Gong, Y. [3 ]
Wang, P. W. [3 ]
Guttman, D. S. [4 ]
Waters, V. [1 ,5 ]
机构
[1] Hosp Sick Children, Res Inst, Translat Med, 555 Univ Ave, Toronto, ON M5G 1X8, Canada
[2] Univ Toronto, Hosp Sick Children, Div Microbiol, Dept Pediat Lab Med, 555 Univ Ave, Toronto, ON M5G 1X8, Canada
[3] Univ Toronto, Ctr Anal Genome Evolut & Funct, Toronto, ON, Canada
[4] Univ Toronto, Dept Cell & Syst Biol, Toronto, ON, Canada
[5] Univ Toronto, Hosp Sick Children, Div Infect Dis, Dept Pediat, 555 Univ Ave, Toronto, ON M5G 1X8, Canada
关键词
BACTERIAL BIOFILMS; ERADICATION; CHILDREN; PSL; EXOPOLYSACCHARIDE; FAILURE; ROLES;
D O I
10.1038/s41522-017-0035-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Antimicrobial resistance is a significant threat to the treatment of infectious disease. Multiple mechanisms of resistance to different classes of antibiotics have been identified and well-studied. However, these mechanisms are studied with bacteria in isolation, whereas often, infections have a polymicrobial basis. Using a biofilm slide chamber model, we visualized the formation and development of clinical Pseudomonas aeruginosa biofilms in the presence of secreted Staphylococcus aureus exoproducts, two bacteria that commonly co-infect pediatric patients with cystic fibrosis. We showed that, over time, certain isolates of P. aeruginosa can form different biofilm architecture in the presence of S. aureus exoproducts. We further determined that this interaction was dependent on Psl produced by P. aeruginosa and staphylococcal protein A from S. aureus. Importantly, we identified a mechanism of antibiotic resistance to tobramycin that is dependent on the polymicrobial interactions between these two bacteria. This interaction occurred in isolates of P. aeruginosa recovered from children with cystic fibrosis who failed to clear P. aeruginosa following inhaled tobramycin treatment.
引用
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页数:9
相关论文
共 37 条
[1]   Staphylococcus aureus Protein A Mediates Interspecies Interactions at the Cell Surface of Pseudomonas aeruginosa [J].
Armbruster, Catherine R. ;
Wolter, Daniel J. ;
Mishra, Meenu ;
Hayden, Hillary S. ;
Radey, Matthew C. ;
Merrihew, Gennifer ;
MacCoss, Michael J. ;
Burns, Jane ;
Wozniak, Daniel J. ;
Parsek, Matthew R. ;
Hoffman, Lucas R. .
MBIO, 2016, 7 (03)
[2]   Purification and Characterization of Biofilm-Associated EPS Exopolysaccharides from ESKAPE Organisms and Other Pathogens [J].
Bales, Patrick M. ;
Renke, Emilija Miljkovic ;
May, Sarah L. ;
Shen, Yang ;
Nelson, Daniel C. .
PLOS ONE, 2013, 8 (06)
[3]  
BEAUDOIN T, 2016, JOVE J VIS EXP, DOI DOI 10.3791/54819
[4]   The Extracellular Matrix Component Psl Provides Fast-Acting Antibiotic Defense in Pseudomonas aeruginosa Biofilms [J].
Billings, Nicole ;
Millan, Maria Ramirez ;
Caldara, Marina ;
Rusconi, Roberto ;
Tarasova, Yekaterina ;
Stocker, Roman ;
Ribbeck, Katharina .
PLOS PATHOGENS, 2013, 9 (08)
[5]   Effectiveness of a stepwise Pseudomonas aeruginosa eradication protocol in children with cystic fibrosis [J].
Blanchard, Ana C. ;
Horton, Eric ;
Stanojevic, Sanja ;
Taylor, Louise ;
Waters, Valerie ;
Ratjen, Felix .
JOURNAL OF CYSTIC FIBROSIS, 2017, 16 (03) :395-400
[6]   Pseudomonas aeruginosa uses a cyclic-di-GMP-regulated adhesin to reinforce the biofilm extracellular matrix [J].
Borlee, Bradley R. ;
Goldman, Aaron D. ;
Murakami, Keiji ;
Samudrala, Ram ;
Wozniak, Daniel J. ;
Parsek, Matthew R. .
MOLECULAR MICROBIOLOGY, 2010, 75 (04) :827-842
[7]   Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production [J].
Byrd, Matthew S. ;
Sadovskaya, Irina ;
Vinogradov, Evgueny ;
Lu, Haiping ;
Sprinkle, April B. ;
Richardson, Stephen H. ;
Ma, Luyan ;
Ralston, Brad ;
Parsek, Matthew R. ;
Anderson, Erin M. ;
Lam, Joseph S. ;
Wozniak, Daniel J. .
MOLECULAR MICROBIOLOGY, 2009, 73 (04) :622-638
[8]   Enhanced In Vitro Formation and Antibiotic Resistance of Nonattached Pseudomonas aeruginosa Aggregates through Incorporation of Neutrophil Products [J].
Caceres, Silvia M. ;
Malcolm, Kenneth C. ;
Taylor-Cousar, Jennifer L. ;
Nichols, David P. ;
Saavedra, Milene T. ;
Bratton, Donna L. ;
Moskowitz, Samuel M. ;
Burns, Jane L. ;
Nick, Jerry A. .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2014, 58 (11) :6851-6860
[9]   Dynamic Remodeling of Microbial Biofilms by Functionally Distinct Exopolysaccharides [J].
Chew, Su Chuen ;
Kundukad, Binu ;
Seviour, Thomas ;
van der Maarel, Johan R. C. ;
Yang, Liang ;
Rice, Scott A. ;
Doyle, Patrick ;
Kjelleberg, Staffan .
MBIO, 2014, 5 (04) :1-11
[10]   Bacterial biofilms: A common cause of persistent infections [J].
Costerton, JW ;
Stewart, PS ;
Greenberg, EP .
SCIENCE, 1999, 284 (5418) :1318-1322