Influence of clove oil on certain quorum-sensing-regulated functions and biofilm of Pseudomonas aeruginosa and Aeromonas hydrophila

被引:116
作者
Husain, Fohad Mabood [1 ]
Ahmad, Iqbal [1 ]
Asif, Mohammad [2 ]
Tahseen, Qudsia [2 ]
机构
[1] Aligarh Muslim Univ, Dept Agr Microbiol, Aligarh 202002, Uttar Pradesh, India
[2] Aligarh Muslim Univ, Dept Zool, Aligarh 202002, Uttar Pradesh, India
关键词
Biofilm; C; elegans; clove oil; quorum-sensing inhibition; VIRULENCE FACTORS; MEDICINAL-PLANTS; CAENORHABDITIS-ELEGANS; CELL COMMUNICATION; INHIBITION; IDENTIFICATION; EXPRESSION; SIGNALS; EUGENOL; SYSTEM;
D O I
10.1007/s12038-013-9385-9
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Quorum sensing (QS) plays an important role in virulence, biofilm formation and survival of many pathogenic bacteria including Pseudomonas aeruginosa. This signalling pathway is considered as novel and promising target for anti-infective agents. In the present investigation, effect of the Sub-MICs of clove oil on QS regulated virulence factors and biofilm formation was evaluated against P. aeruginosa PAO1 and Aeromonas hydrophila WAF-38 strain. Sub-inhibitory concentrations of the clove oil demonstrated statistically significant reduction of las- and rhl-regulated virulence factors such as LasB, total protease, chitinase and pyocyanin production, swimming motility and exopolysaccharide production. The biofilm forming capability of PAO1 and A. hydrophila WAF-38 was also reduced in a concentration-dependent manner at all tested sub-MIC values. Further, the PAO1-preinfected Caenorhabditis elegans displayed an enhanced survival when treated with 1.6% v/v of clove oil. The above findings highlight the promising anti-QS-dependent therapeutic function of clove oil against P. aeruginosa.
引用
收藏
页码:835 / 844
页数:10
相关论文
共 54 条
[1]   Attenuation of Pseudomonas aeruginosa virulence by medicinal plants in a Caenorhabditis elegans model system [J].
Adonizio, Allison ;
Leal, Sixto M., Jr. ;
Ausubel, Frederick M. ;
Mathee, Kalai .
JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 57 (07) :809-813
[2]   Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by south Florida plant extracts [J].
Adonizio, Allison ;
Kong, Kok-Fai ;
Mathee, Kalai .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (01) :198-203
[3]   Anti-quorum sensing activity of medicinal plants in southern Florida [J].
Adonizio, Allison L. ;
Downum, Kelsey ;
Bennett, Bradley C. ;
Mathee, Kalai .
JOURNAL OF ETHNOPHARMACOLOGY, 2006, 105 (03) :427-435
[4]  
Ahmad I., 2008, Plant-Bacteria Interactions: Strategies and Techniques to Promote Plant Growth, P129
[5]   Inhibition of quorum sensing in Pseudomonas aeruginosa by azithromycin and its effectiveness in urinary tract infections [J].
Bala, Anju ;
Kumar, Ravi ;
Harjai, Kusum .
JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 60 (03) :300-306
[6]  
Clinical and Laboratory Standards Institute, 2007, M100S17 CLSI
[7]   A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS [J].
DUBOIS, M ;
GILLES, K ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
NATURE, 1951, 168 (4265) :167-167
[8]   IDENTIFICATION AND CHARACTERIZATION OF GENES FOR A 2ND ANTHRANILATE SYNTHASE IN PSEUDOMONAS-AERUGINOSA - INTERCHANGEABILITY OF THE 2 ANTHRANILATE SYNTHASES AND EVOLUTIONARY IMPLICATIONS [J].
ESSAR, DW ;
EBERLY, L ;
HADERO, A ;
CRAWFORD, IP .
JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :884-900
[9]   Regulation of gene expression by cell-to-cell communication: Acyl-homoserine lactone quorum sensing [J].
Fuqua, C ;
Parsek, MR ;
Greenberg, EP .
ANNUAL REVIEW OF GENETICS, 2001, 35 :439-468
[10]   Pseudomonas aeruginosa PAO1 kills Caenorhabditis elegans by cyanide poisoning [J].
Gallagher, LA ;
Manoil, C .
JOURNAL OF BACTERIOLOGY, 2001, 183 (21) :6207-6214