Enantiomeric resolution of drugs and metabolites in polysaccharide- and protein-based chiral stationary phases

被引:24
作者
Bonato, PS
Bortocan, R
Gaitani, CM
Paias, FO
Iha, MH
Lima, RP
机构
[1] Univ Sao Paulo, Fac Ciencias Farmaceut, BR-14040903 Ribeirao Preto, Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, BR-14040903 Ribeirao Preto, Brazil
[3] Inst Adolfo Lutz Registro, Lab Ribeirao Preto 1, BR-14085410 Ribeirao Preto, Brazil
关键词
polysaccharide-based stationary phases; protein-based stationary phases; drugs; metabolites; enantioselective analysis;
D O I
10.1590/S0103-50532002000200010
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Several chiral stationary phases based on polysaccharide derivatives and proteins were evaluated for the resolution of some chiral drugs and their metabolites. The polysaccharide-based stationary phases CHIRALCEL OD-H, CHIRALCEL OB-H, CHIRALCEL OJ, CHIRALPAK AD and CHIRALPAK AS were evaluated under normal phase conditions, using hexane: 2-propanol or hexane: ethanol as mobile phase. Diethylamine and trifluoracetic acid were also added to improve peak shape. The CHIRALCEL OJ-R, CHIRALCEL OD-R and CHIRALCEL OD-H columns were evaluated under reversed-phase conditions, using acetonitrile:H2O or acetonitrile: NaClO4 solution. The protein-based stationary phases, CHIRAL AGP and ULTRON ES-OVM columns were used with mobile phases consisting of a buffer solution supplemented with an organic modifier. Among the polysaccharide-based stationary phases, CHIRALPAK AD provided better resolution for almost all drugs and metabolites studied. The ULTRON ES-OVM column was particularly suitable for the resolution of the four enantiomers of thioridazine-2-sulfoxide.
引用
收藏
页码:190 / 199
页数:10
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