Development of UV-Excitable Red and Near-Infrared Fluorescent Labels and Their Application for Simultaneous Multicolor Bioimaging by Single-Wavelength Excitation

被引：10

作者：

Mizuno, Tetsuya ^{[1
]}

Umezawa, Keitaro ^{[2
]}

Shindo, Yutaka ^{[2
]}

Citterio, Daniel ^{[1
]}

Oka, Kotaro ^{[2
]}

Suzuki, Koji ^{[1
]}

机构：

[1] Keio Univ, Fac Sci & Technol, Dept Appl Chem, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan

[2] Keio Univ, Fac Sci & Technol, Dept Bioinformat, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan

来源：

JOURNAL OF FLUORESCENCE | 2013年 / 23卷 / 05期

关键词：

Bioimaging; BODIPY; Fluorescent dyads; Near-infrared fluorophore; ENERGY-TRANSFER CASSETTES; DYES; PROTEIN; CHEMISTRY; EMISSION; ABSORPTION; MOLECULES; ACCEPTOR; SENSORS; BRIGHT;

D O I：

10.1007/s10895-013-1227-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a new type of UV-excitable red/NIR-emissive fluorescent dyads (PKF series). Conjugation of a pyrene and a novel bright red/near-infrared (NIR) fluorophore resulted in large quasi-Stokes shift while retaining intense fluorescence emission and sharp spectral bands. Labeling of PKF dyads to biomolecules was performed by means of introduction of a succinimidyl ester. Simultaneous Ca2+/albumin dual-color intracellular imaging by PKF in combination with fura-2 (UV-excitable/VIS-emissive Ca2+ indicator) reveals its usefulness as a new bioimaging tool.

引用

页码：1007 / 1018

页数：12

共 4 条

[1] Development of UV-Excitable Red and Near-Infrared Fluorescent Labels and Their Application for Simultaneous Multicolor Bioimaging by Single-Wavelength Excitation
Tetsuya Mizuno
Keitaro Umezawa
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Daniel Citterio
Kotaro Oka
Koji Suzuki
Journal of Fluorescence, 2013, 23 : 1007 - 1018
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[4] Rational design of far red to near-infrared rhodamine analogues with huge Stokes shifts for single-laser excitation multicolor imaging
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