Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma

被引：61

作者：

Farrera-Soler, Lluc ^{[1
]}

Daguer, Jean-Pierre ^{[1
]}

Barluenga, Sofia ^{[1
]}

Vadas, Oscar ^{[2
]}

Cohen, Patrick ^{[3
,4
]}

Pagano, Sabrina ^{[3
,4
]}

Yerly, Sabine ^{[3
,4
]}

Kaiser, Laurent ^{[3
,4
,5
]}

Vuilleumier, Nicolas ^{[3
,4
]}

Winssinger, Nicolas ^{[1
]}

机构：

[1] Univ Geneva, Fac Sci, Dept Organ Chem, NCCR Chem Biol, Geneva, Switzerland

[2] Univ Geneva, Dept Microbiol & Mol Med, Geneva, Switzerland

[3] Geneva Univ Hosp, Diagnost Dept, Div Lab Med, Geneva, Switzerland

[4] Fac Med, Geneva, Switzerland

[5] Geneva Univ Hosp, Div Infect Dis, Geneva, Switzerland

来源：

PLOS ONE | 2020年 / 15卷 / 09期

关键词：

CORONAVIRUS SPIKE PROTEIN; SYNDROME SARS CORONAVIRUS; S2; DOMAIN; ANTIBODIES; VIRUS; IMMUNIZATION; RESPONSES; SITE; PNA;

D O I：

10.1371/journal.pone.0238089

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we identified three immunodominant linear epitopes, two of which correspond to key proteolytic sites on the spike protein (S1/S2 and S2') known to be critical for cellular entry. We show biochemical evidence that plasma positive for the epitope adjacent to the S1/S2 cleavage site inhibits furin-mediated proteolysis of spike.

引用

页数：15

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