Enhanced performance in the determination of ibuprofen 1-β-O-acyl glucuronide in urine by combining high field asymmetric waveform ion mobility spectrometry with liquid chromatography-time-of-flight mass spectrometry

被引：17

作者：

Smith, Robert W. ^{[1
]}

Toutoungi, Danielle E. ^{[2
]}

Reynolds, James C. ^{[1
]}

Bristow, Anthony W. T. ^{[3
]}

Ray, Andrew ^{[3
]}

Sage, Ashley ^{[4
]}

Wilson, Ian D. ^{[5
]}

Weston, Daniel J. ^{[5
]}

Boyle, Billy ^{[2
]}

Creaser, Colin S. ^{[1
]}

机构：

[1] Univ Loughborough, Dept Chem, Ctr Analyt Sci, Loughborough LE11 3TU, Leics, England

[2] Owlstone Ltd, Cambridge CB4 0GD, England

[3] AstraZeneca, Pharmaceut Dev, Macclesfield SK10 2NA, Cheshire, England

[4] Agilent Technol, Stockport SK8 3GR, Lancs, England

[5] AstraZeneca R&D, DMPK Innovat Med, Macclesfield SK10 4TG, Cheshire, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2013年 / 1278卷

关键词：

UHPLC; FAIMS; Mass spectrometry; Metabolite; Urine; ELECTROSPRAY; SEPARATION; DISSOCIATION; HRMS;

D O I：

10.1016/j.chroma.2012.12.065

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The incorporation of a chip-based high field asymmetric waveform ion mobility spectrometry (FAIMS) separation in the ultra (high)-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) determination of the (R/S) ibuprofen 1-beta-O-acyl glucuronide metabolite in urine is reported. UHPLC-FAIMS-HRMS reduced matrix chemical noise, improved the limit of quantitation approximately two-fold and increased the linear dynamic range compared to the determination of the metabolite without FAIMS separation. A quantitative evaluation of the prototype UHPLC-FAIMS-HRMS system showed better reproducibility for the drug metabolite (%RSD 2.7%) at biologically relevant concentrations in urine. In-source collision induced dissociation of the FAIMS-selected deprotonated metabolite was used to fragment the ion prior to mass analysis, enhancing selectivity by removing co-eluting species and aiding the qualitative identification of the metabolite by increasing the signal-to-noise ratio of the fragment ions. (c) 2013 Elsevier B.V. All rights reserved.

引用

页码：76 / 81

页数：6

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