Extracellular Vesicles from Skeletal Muscle Cells Efficiently Promote Myogenesis in Induced Pluripotent Stem Cells

被引:15
|
作者
Baci, Denisa [1 ,2 ]
Chirivi, Maila [1 ]
Pace, Valentina [1 ]
Maiullari, Fabio [3 ]
Milan, Marika [1 ]
Rampin, Andrea [1 ]
Somma, Paolo [4 ]
Presutti, Dario [1 ]
Garavelli, Silvia [5 ]
Bruno, Antonino [6 ]
Cannata, Stefano [7 ]
Lanzuolo, Chiara [8 ,9 ]
Gargioli, Cesare [7 ]
Rizzi, Roberto [8 ,9 ]
Bearzi, Claudia [1 ,9 ]
机构
[1] CNR, Inst Biochem & Cell Biol, I-00015 Rome, Italy
[2] Univ Insubria, Dept Biotechnol & Life Sci, I-21100 Varese, Italy
[3] Gemelli Molise Hosp, I-86100 Campobasso, Italy
[4] Humanitas Clin & Res Ctr, Flow Cytometry Core, I-20089 Milan, Italy
[5] CNR, Inst Endocrinol & Oncol Gaetano Salvatore, I-80131 Naples, Italy
[6] IRCCS MultiMed, I-20138 Milan, Italy
[7] Univ Roma Tor Vergata, Dept Biol, I-00133 Rome, Italy
[8] CNR, Inst Biomed Technol, I-20090 Milan, Italy
[9] Fdn Ist Nazl Genet Mol, I-20122 Milan, Italy
关键词
iPSC; extracellular vesicles; pericytes; skeletal muscle; IPS CELLS; DIFFERENTIATION; MYOBLASTS; EXOSOMES; PRECURSORS; DERIVATION; MICRORNAS; PERICYTES; IDENTIFICATION; PROGENITORS;
D O I
10.3390/cells9061527
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The recent advances, offered by cell therapy in the regenerative medicine field, offer a revolutionary potential for the development of innovative cures to restore compromised physiological functions or organs. Adult myogenic precursors, such as myoblasts or satellite cells, possess a marked regenerative capacity, but the exploitation of this potential still encounters significant challenges in clinical application, due to low rate of proliferation in vitro, as well as a reduced self-renewal capacity. In this scenario, induced pluripotent stem cells (iPSCs) can offer not only an inexhaustible source of cells for regenerative therapeutic approaches, but also a valuable alternative for in vitro modeling of patient-specific diseases. In this study we established a reliable protocol to induce the myogenic differentiation of iPSCs, generated from pericytes and fibroblasts, exploiting skeletal muscle-derived extracellular vesicles (EVs), in combination with chemically defined factors. This genetic integration-free approach generates functional skeletal myotubes maintaining the engraftment ability in vivo. Our results demonstrate evidence that EVs can act as biological "shuttles" to deliver specific bioactive molecules for a successful transgene-free differentiation offering new opportunities for disease modeling and regenerative approaches.
引用
收藏
页码:1 / 21
页数:21
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