Genetic variation in histidine rich proteins among Indian Plasmodium falciparum population: possible cause of variable sensitivity of malaria rapid diagnostic tests

被引：44

作者：

Kumar, Navin ^{[1
]}

Singh, Jai P. N. ^{[1
]}

Pande, Veena ^{[2
]}

Mishra, Neelima ^{[1
]}

Srivastava, Bina ^{[1
]}

Kapoor, Ridhima ^{[1
]}

Valecha, Neena ^{[1
]}

Anvikar, Anupkumar R. ^{[1
]}

机构：

[1] Natl Inst Malaria Res ICMR, Sect 8, New Delhi 110077, India

[2] Kumaun Univ, Dept Biotechnol, Naini Tal, Uttarakhand, India

来源：

MALARIA JOURNAL | 2012年 / 11卷

关键词：

Plasmodium falciparum Histidine rich protein 2; Plasmodium falciparum Histidine rich protein 3; Rapid Diagnostic Tests; Genetic polymorphism; India; SEQUENCE VARIATION; PERFORMANCE; PFHRP2; ANTIBODIES; DIVERSITY; PARASITES;

D O I：

10.1186/1475-2875-11-298

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background: Rapid diagnostic tests (RDTs) have revolutionized the diagnosis of malaria. Among the various factors affecting RDTs sensitivity is genetic variation of the antigen used. The genetic variation in PfHRP2 and PfHRP3 proteins was studied among the Indian Plasmodium falciparum isolates. Methods: One hundred and forty isolates of P. falciparum were collected from six geographical regions of India. Target genes encoding PfHRP2 and PfHRP3 antigens were sequenced to study genetic polymorphism. Minimum detection limit giving a positive rapid diagnostic test was also determined. Results: Extensive variations were observed in amino acid repeat types of PfHRP2 and PfHRP3. PfHRP2 exhibited more polymorphism than PfHRP3. Significant relation was observed between type 2 and type 7 repeats and RDT detection rate as higher number of these repeats showed better sensitivity with RDTs. Conclusion: The results provide insights into the genetic diversity of Pfhrp2 and Pfhrp3 genes among Indian P. falciparum population and its relation to RDT sensitivity.

引用

页数：7