Genetic engineering of wheat for increased resistance to powdery mildew disease

被引：108
作者：
Bliffeld, M
Mundy, J
Potrykus, I
Fütterer, J
机构：
[1] ETH Zurich, Inst Plant Sci, CH-8092 Zurich, Switzerland
[2] Inst Mol Biol, Dept Plant Physiol, DK-1353 Copenhagen K, Denmark
来源：
THEORETICAL AND APPLIED GENETICS | 1999年 / 98卷 / 6-7期
关键词：
antifungal proteins; chitinase; biolistic transformation; wheat; chlorophenolred; phosphinotricin; Erysiphe graminis;
D O I：
10.1007/s001220051170
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Fungal wheat (Triticum aestivum) diseases greatly affect crop productivity and require the economically and ecologically undesirable application of fungicides in wheat agriculture. We have generated transgenic wheat plants constitutively expressing an antifungal barley-seed class II chitinase. The transgene was stably expressed and the chitinase properly localized in the apoplast of the transgenic lines. The engineered wheat plants showed increased resistance to infection with the powdery mildew-causing fungus Erysiphe graminis.
引用
收藏
页码：1079 / 1086
页数：8
相关论文
共 46 条
[1]  
[Anonymous], 1994, Crop production and crop protection: estimated losses in major food and cash crops
[2]   Comparison between QTL analysis of powdery mildew resistance in barley based on detached primary leaves and on field data [J].
Backes, G ;
Schwarz, G ;
Wenzel, G ;
Jahoor, A .
PLANT BREEDING, 1996, 115 (05) :419-421
[3]   CHITIN OLIGOSACCHARIDES ELICIT LIGNIFICATION IN WOUNDED WHEAT LEAVES [J].
BARBER, MS ;
BERTRAM, RE ;
RIDE, JP .
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 1989, 34 (01) :3-12
[4]   ANTIFUNGAL EFFECT OF BEAN ENDOCHITINASE ON RHIZOCTONIA-SOLANI - ULTRASTRUCTURAL-CHANGES AND CYTOCHEMICAL ASPECTS OF CHITIN BREAKDOWN [J].
BENHAMOU, N ;
BROGLIE, K ;
BROGLIE, R ;
CHET, I .
CANADIAN JOURNAL OF MICROBIOLOGY, 1993, 39 (03) :318-328
[5]   THE RELATIONSHIP BETWEEN THE EXPRESSION OF DEFENSE-RELATED GENES AND MILDEW DEVELOPMENT IN BARLEY [J].
BOYD, LA ;
SMITH, PH ;
GREEN, RM ;
BROWN, JKM .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1994, 7 (03) :401-410
[6]   CHARACTERIZATION OF A PLANT SCAFFOLD ATTACHMENT REGION IN A DNA FRAGMENT THAT NORMALIZES TRANSGENE EXPRESSION IN TOBACCO [J].
BREYNE, P ;
VANMONTAGU, M ;
DEPICKER, A ;
GHEYSEN, G .
PLANT CELL, 1992, 4 (04) :463-471
[7]   TRANSGENIC PLANTS WITH ENHANCED RESISTANCE TO THE FUNGAL PATHOGEN RHIZOCTONIA-SOLANI [J].
BROGLIE, K ;
CHET, I ;
HOLLIDAY, M ;
CRESSMAN, R ;
BIDDLE, P ;
KNOWLTON, S ;
MAUVAIS, CJ ;
BROGLIE, R .
SCIENCE, 1991, 254 (5035) :1194-1197
[8]   MAIZE POLYUBIQUITIN GENES - STRUCTURE, THERMAL PERTURBATION OF EXPRESSION AND TRANSCRIPT SPLICING, AND PROMOTER ACTIVITY FOLLOWING TRANSFER TO PROTOPLASTS BY ELECTROPORATION [J].
CHRISTENSEN, AH ;
SHARROCK, RA ;
QUAIL, PH .
PLANT MOLECULAR BIOLOGY, 1992, 18 (04) :675-689
[9]   DIFFERENTIAL EXPRESSION AND INDUCTION OF CHITINASES AND BETA-1,3-GLUCANASES IN RESPONSE TO FUNGAL INFECTION DURING GERMINATION OF MAIZE SEEDS [J].
CORDERO, MJ ;
RAVENTOS, D ;
SANSEGUNDO, B .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1994, 7 (01) :23-31
[10]   OLIGOSACCHARINS - STRUCTURES AND SIGNAL-TRANSDUCTION [J].
COTE, F ;
HAHN, MG .
PLANT MOLECULAR BIOLOGY, 1994, 26 (05) :1379-1411
12345