Cleavage and protection of locked nucleic acid-modified DNA by restriction endonucleases

被引：9

作者：

Crouzier, Lucile ^{[1
,2
]}

Dubois, Camille ^{[1
,2
]}

Wengel, Jesper ^{[3
]}

Veedu, Rakesh N. ^{[1
]}

机构：

[1] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia

[2] Inst Polytech LaSalle Beauvais, F-60026 Beauvais, France

[3] Univ So Denmark, Dept Phys Chem & Pharm, Nucle Acid Ctr, DK-5230 Odense M, Denmark

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2012年 / 22卷 / 14期

基金：

欧洲研究理事会;

关键词：

Modified nucleic acids; Locked nucleic acids; Oligonucleotides; PHOTO-CROSS-LINKING; ENDODEOXYRIBONUCLEASE ECORI; RECOGNITION; ANALOGS; OLIGODEOXYNUCLEOTIDES; DUPLEXES; TAQI; LNA; METHYLTRANSFERASE; OLIGONUCLEOTIDES;

D O I：

10.1016/j.bmcl.2012.05.113

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far. We herein for the first time report cleavage by restriction endonuclease of LNA-modified DNA oligonucleotides. The experiments revealed that RsaI is an efficient enzyme capable of recognizing and cleaving LNA-modified DNA oligonucleotides. Furthermore, introduction of LNA nucleotides protects against cleavage by the restriction endonucleases PvuII, PstI, SacI, KpnI and EcoRI. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：4836 / 4838

页数：3

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