Analysis of orthologous hrcA genes in Escherichia coli and Bacillus subtilis

被引：3

作者：

Wiegert, T ^{[1
]}

Hagmaier, K ^{[1
]}

Schumann, W ^{[1
]}

机构：

[1] Univ Bayreuth, Inst Genet, D-95440 Bayreuth, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2004年 / 234卷 / 01期

关键词：

heat shock; hrcA; GroE chaperonin system; bgaB reporter gene;

D O I：

10.1016/j.femsle.2004.02.017

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The hrcA gene codes fora transcriptional repressor protein interacting with the CIRCE operator thereby reducing expression of the aroE operon of more than 120 bacterial species. At least in Bacillus subtilis, the activity of the HrcA protein is modulated by the GroE chaperonin system. We amplified the hrcA gene from five different bacterial species and analyzed its activity in Escherichia coli and Bacillus subtilis. While those from Clostridium acetobutylicum and Staphylococcus aureus turned out to be active, those of Helicobacter pylori, Lactococcus lactis and Thermotoga maritima were inactive in E. coli, but that of T. maritima turned out to repress expression of the reporter gene in B. subtilis. All these results strongly suggest to us a specific recognition of HrcA by the GroE chaperonin system. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：9 / 17

页数：9

共 35 条

[1] A protein-based phylogenetic tree for Gram-positive bacteria derived from hrcA, a unique heat-shock regulatory gene [J].