Gonadotrophs in long-term ovariectomized rat females treated with luteinizing hormone releasing hormone and estradiol

被引：0

作者：

Lovren, M ^{[1
]}

Sekulic, M ^{[1
]}

Milosevic, V ^{[1
]}

Radulovic, N ^{[1
]}

机构：

[1] Inst Biol Res Sinisa Stankovic, Belgrade, Yugoslavia

来源：

ACTA VETERINARIA-BEOGRAD | 1999年 / 49卷 / 2-3期

关键词：

gonadotrophic cells; ovariectomy; LHRH; EDP; immunohistochemistry; stereology;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The effects of multiple estradiol dipropionate (EDP) treatment, high doses of luteinizing hormone releasing hormone (LHRH) and the combination of both on immunohistochemical and morphometric parameters of gonadotrophic cells in long-term ovariectomized (ovx) rats were studied using rabbit anti-rat beta-follicle stimulating hormone beta-FSH) and beta-luteinizing hormone (beta-LH) sera and a peroxidase-antiperoxidase (PAP) immunohistochemical procedure. A morphometric method for determining changes of gonadotrophic cell volume, as well as of their number and relative volume density was used. All examined morphometric values in treated females showed a significant decrease in comparison with the ovx controls, the most significant decrease being observed in EDP and EDP+LHRH-treated rats. These results indicate that besides inhibitory action of LHRH and a singificant inhibitory effect of EDF: the combination of both expressed a prominent inhibitory effect on the examined parameters of gonadotrophic cells in long-term ovx females.

引用

页码：117 / 126

页数：10

共 30 条

[1] GONADOTROPIN-RELEASING-HORMONE (GNRH) RECEPTOR DYNAMICS AND GONADOTROPE RESPONSIVENESS DURING AND AFTER CONTINUOUS GNRH STIMULATION [J].