Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing

被引：10

作者：

Park, Hanseul ^{[1
,2
]}

Shin, Jaein ^{[1
,2
]}

Choi, Hwan ^{[1
,2
]}

Cho, Byounggook ^{[1
,2
]}

Kim, Jongpil ^{[1
,2
]}

机构：

[1] Dongguk Univ, Dept Biomed Engn BK21 Plus, Seoul 04620, South Korea

[2] Dongguk Univ, Dept Chem, Lab Cell Reprogramming & Gene Editing, Seoul 04620, South Korea

来源：

CELLS | 2020年 / 9卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

embryo; gene editing; CRISPR; Cas9; valproic acid; PLURIPOTENT STEM-CELLS; IN-VITRO DEVELOPMENT; HISTONE DEACETYLASE; CAS9; PROTEIN; ACTIVATION; DIFFERENTIATION; EXPRESSION; APOPTOSIS; RECEPTOR; MICE;

D O I：

10.3390/cells9061447

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has emerged as a powerful technology, with the potential to generate transgenic animals. Particularly, efficient and precise genetic editing with CRISPR/Cas9 offers immense prospects in various biotechnological applications. Here, we report that the histone deacetylase inhibitor valproic acid (VPA) significantly increases the efficiency of CRISPR/Cas9-mediated gene editing in mouse embryonic stem cells and embryos. This effect may be caused through globally enhanced chromatin accessibility, as indicate by histone hyperacetylation. Taken together, our results suggest that VPA can be used to increase the efficacy of CRISPR/Cas9 in generating transgenic systems.

引用

页码：1 / 13

页数：13

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