Self-digitization chip for quantitative detection of human papillomavirus gene using digital LAMP

被引：44

作者：

Kreutz, Jason E. ^{[1
]}

Wang, Jiasi ^{[1
]}

Sheen, Allison M. ^{[1
]}

Thompson, Alison M. ^{[2
]}

Staheli, Jeannette P. ^{[3
]}

Dyen, Michael R. ^{[3
]}

Feng, Qinghua ^{[4
]}

Chiu, Daniel T. ^{[1
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

[2] Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA

[3] Seattle Childrens Res Inst, Ctr Global Infect Dis Res, Seattle, WA USA

[4] FIDALAB, Seattle, WA USA

来源：

LAB ON A CHIP | 2019年 / 19卷 / 06期

基金：

比尔及梅琳达.盖茨基金会; 美国国家卫生研究院;

关键词：

MEDIATED ISOTHERMAL AMPLIFICATION; POLYMERASE-CHAIN-REACTION; ABSOLUTE QUANTIFICATION; VISUAL DETECTION; MESSENGER-RNA; PCR; SURVIVAL; CANCER; POINT; HPV;

D O I：

10.1039/c8lc01223g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Digital nucleic acid amplification and detection methods provide excellent sensitivity and specificity and allow absolute quantification of target nucleic acids. Isothermal methods such as digital loop-mediated isothermal amplification (digital LAMP) have potential for use in rapid disease diagnosis in low-resource settings due to their speed and lack of thermal cycling. We previously developed a self-digitization (SD) chip, a simple microfluidics device that automatically digitizes a sample into an array of nanoliter wells, for use in digital LAMP. In this work, we improve the SD chip design to increase sample loading efficiency, speed, and completeness, and test a range of well volumes and numbers. We demonstrate the diagnostic capability of this platform by applying it to quantifying human papillomavirus 18 gene.

引用

页码：1035 / 1040

页数：6

共 35 条

[1] Human Papillomavirus and Survival of Patients with Oropharyngeal Cancer [J].