Trimodal spectroscopy for the detection and characterization of cervical precancers in vivo

被引：185

作者：

Georgakoudi, I

Sheets, EE

Müller, MG

Backman, V

Crum, CP

Badizadegan, K

Dasari, RR

Feld, MS

机构：

[1] MIT, Spect Lab, Cambridge, MA 02139 USA

[2] Brigham & Womens Hosp, Dept Obstet & Gynecol, Div Gynecol Oncol, Boston, MA 02115 USA

[3] Brigham & Womens Hosp, Dept Pathol, Div Womens & Perinatal Pathol, Boston, MA 02115 USA

[4] Childrens Hosp, Dept Pathol, Boston, MA 02115 USA

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 2002年 / 186卷 / 03期

关键词：

cancer; diagnostic; spectroscopy; fluorescence; diffuse reflectance; light scattering; cervical neoplasia;

D O I：

10.1067/mob.2002.121075

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

OBJECTIVE: The objective of this study was to assess the potential of 3 spectroscopic techniques (intrinsic fluorescence, diffuse reflectance, and light scattering) individually and in combination (trimodal spectroscopy) for the detection of cervical squamous intraepithelial lesions. STUDY DESIGN: The study was conducted with 44 patients who underwent colposcopy for the evaluation of an abnormal Papanicolaou smear. Fluorescence and reflectance spectra were collected from colposcopically normal and abnormal sites and analyzed to extract quantitative information about tissue biochemistry and morphologic condition. This information was compared with histopathologic classification, and diagnostic algorithms were developed and validated with the use of logistic regression and cross-validation. RESULTS: Diagnostically significant differences exist in the composition of fluorescing biochemicals, the scattering properties, and the epithelial cell nuclear morphology of cervical squamous intraepithelial lesions and non-squamous intraepithelial lesions. Trimodal spectroscopy is a superior tool for the detection of cervical squamous intraepithelial lesions than any 1 of the techniques alone. CONCLUSION: Trimodal spectroscopy has the potential to improve the in vivo detection of precancerous cervical changes.

引用

页码：374 / 382

页数：9

共 28 条

[11]

MITCHELL MF, 1994, CLIN CONSULTATIONS O, V6, P70

[12] Observation of periodic fine structure in reflectance from biological tissue: A new technique for measuring nuclear size distribution [J].

Perelman, LT ;

Backman, V ;

Wallace, M ;

Zonios, G ;

Manoharan, R ;

Nusrat, A ;

Shields, S ;

Seiler, M ;

Lima, C ;

Hamano, T ;

Itzkan, I ;

Van Dam, J ;

Crawford, JM ;

Feld, MS .

PHYSICAL REVIEW LETTERS, 1998, 80 (03) :627-630

[13]

Ramanujam N, 1996, LASER SURG MED, V19, P63, DOI 10.1002/(SICI)1096-9101(1996)19:1<63::AID-LSM8>3.0.CO

[14]

2-O

[15] Cervical precancer detection using a multivariate statistical algorithm based on laser-induced fluorescence spectra at multiple excitation wavelengths [J].

Ramanujam, N ;

Mitchell, MF ;

MahadevanJansen, A ;

Thomsen, SL ;

Staerkel, G ;

Malpica, A ;

Wright, T ;

Atkinson, N ;

RichardsKortum, R .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1996, 64 (04) :720-735

[16]

Ramanujam N, 1996, LASER SURG MED, V19, P46, DOI 10.1002/(SICI)1096-9101(1996)19:1<46::AID-LSM7>3.3.CO

[17]

2-J

[18] IN-VIVO DIAGNOSIS OF CERVICAL INTRAEPITHELIAL NEOPLASIA USING 337-NM-EXCITED LASER-INDUCED FLUORESCENCE [J].

RAMANUJAM, N ;

MITCHELL, MF ;

MAHADEVAN, A ;

WARREN, S ;

THOMSEN, S ;

SILVA, E ;

RICHARDSKORTUM, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (21) :10193-10197

[19] Fluorescence spectroscopy of neoplastic and non-neoplastic tissues [J].

Ramanujam, N .

NEOPLASIA, 2000, 2 (1-2) :89-117

[20] Quantitative optical spectroscopy for tissue diagnosis [J].

RichardsKortum, R ;

SevickMuraca, E .

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 1996, 47 :555-606

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