Laminin-5γ2 chain expression is associated with tumor cell invasiveness and prognosis of lung squamous cell carcinoma

被引:27
作者
Masuda, Ryota [2 ]
Kijima, Hiroshi [1 ,3 ]
Imamura, Naoko [2 ]
Aruga, Naohiro [2 ]
Nakazato, Kenei [2 ]
Oiwa, Kana [2 ]
Nakano, Takayuki [2 ]
Watanabe, Hajime [2 ]
Ikoma, Yoichiro [2 ]
Tanaka, Makiko [1 ]
Inokuchi, Sadaki [1 ]
Iwazaki, Masayuki [2 ]
机构
[1] Hirosaki Univ, Grad Sch Med, Dept Pathol & Biosci, Hirosaki, Aomori 0368562, Japan
[2] Tokai Univ, Sch Med, Dept Gen Thorac Surg, Isehara, Kanagawa 2591193, Japan
[3] Tokai Univ, Sch Med, Dept Crit Care & Emergency Med, Isehara, Kanagawa 2591193, Japan
来源
BIOMEDICAL RESEARCH-TOKYO | 2012年 / 33卷 / 05期
关键词
GAMMA-2; CHAIN; BASEMENT-MEMBRANE; IMMUNOHISTOCHEMICAL ANALYSIS; COLORECTAL-CARCINOMA; LAMININ CHAIN; CANCER; ADENOCARCINOMAS; MIGRATION; PROTEINS; PATTERN;
D O I
10.2220/biomedres.33.309
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Our previous study showed that tumor budding is a significant indicator of a poor prognosis in lung squamous cell carcinoma patients. Tumor budding-positive (Bud(+)) cases of lung squamous cell carcinoma (SqCC) showed locally aggressive growth, and the positivity was a useful indicator of the lymph node status and prognosis. The present study focused on the clinicopathologic significance of laminin-5 gamma 2 chain expression for local aggressiveness in lung SqCC. Laminin-5 gamma 2 chain immunohistochemical stains in tissue samples were divided into three distinct types: basement membrane (B type; laminin-5 gamma 2 present in basement membrane), cytoplasmic (C type; laminin-5 gamma 2 present in intracellular matrix), and invasive front (F type; laminin-5 gamma 2 present in cytoplasm, and strongly in part of peripheral nest). The F type was more common in Bud(+) cases than tumor budding-negative (Bud(-)) cases; B and C types were less common in Bud(+) cases (P < 0.001). The F type was more closely associated with decreased overall survival than the B and C types (P < 0.001 for both). Univariate analysis showed that the F type could be used to predict tumor size, lymph node metastasis, lymphatic invasion, tumor infiltrative patterns, tumor budding, and laminin-5 gamma 2 chain staining. Multivariate analysis showed that laminin-5 gamma 2 chain staining and tumor budding could be used to predict patient mortality (P < 0.001 and P = 0.005, respectively). The overall survival rate after curative resection was lower in patients with the F/Bud(+) type than in those with B+C/Bud(-) and B+C/Bud(+) types (P < 0.001 for both, log-rank test), and also lower with the F/Bud(-) type than the B+C/Bud(-) type. On the other hand, there was no significant difference between the F/Bud(-) and F/Bud(-) types. In conclusion, both laminin-5 gamma 2 chain staining and tumor budding are associated with tumor cell invasiveness and are independent predictors of mortality in lung SqCC patients.
引用
收藏
页码:309 / 317
页数:9
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