Clinical significance of serum DRAM1 mRNA, ARSA mRNA, hsa-miR-2053 and lncRNA-RP1-86D1.3 axis expression in malignant pleural mesothelioma

被引：23

作者：

Matboli, Marwa ^{[1
]}

Shafei, Ayman E. ^{[2
]}

Ali, Mahmoud A. ^{[2
]}

Gaber, Ahmed I. ^{[3
]}

Galal, Ahmed ^{[3
]}

Tarek, Osama ^{[3
]}

Marei, Mohamed ^{[3
]}

Khairy, Eman ^{[1
]}

El-Khazragy, Nashwa ^{[4
]}

Anber, Nahla ^{[5
]}

Abdel-Rahman, Omar ^{[6
]}

机构：

[1] Ain Shams Univ, Fac Med, Dept Med Biochem & Mol Biol, Cairo, Egypt

[2] Armed Forces Coll Med, Dept Biomed Res, Cairo, Egypt

[3] Armed Forces Coll Med, Cairo, Egypt

[4] Ain Shams Univ, Fac Med, Dept Clin Pathol, Oncol Diagnost Unit, Cairo, Egypt

[5] Mansoura Univ, Emergency Hosp, Biochem, Mansoura, Egypt

[6] Ain Shams Univ, Fac Med, Dept Clin Oncol, Cairo, Egypt

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2019年 / 120卷 / 03期

关键词：

bioinformatics; long noncoding RNA diagnosis; mesothelioma; microRNA; POTENTIAL-BIOMARKER; DISCOVERY; AUTOPHAGY; DIAGNOSIS;

D O I：

10.1002/jcb.27586

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aim and Background Malignant pleural mesothelioma (MPM) is a lethal cancer mainly caused by chronic exposure of asbestos. In this pilot study, we aimed to assess the expression of serum RNA-based biomarker panel exploring their clinical utility as diagnostic and prognostic biomarkers for MPM. Methods We have selected an MPM-specific RNA-based biomarker panel through bioinformatics analysis based on the integration of DNA damage regulated autophagy modulator 1 (DRAM1) and arylsulfatase A (ARSA) gene expression with their epigenetic regulators microRNA (miR-2053) and long noncoding RNA (lncRNA-RP1-86D1.3). Then, quantitative real-time polymerase chain reaction (qPCR) validation in sera of 60 MPM patients, 20 chronic asbestos exposure patients, and 20 healthy volunteers was done. Lastly, the prognostic power of the selected panel was assessed. Results The expression of serum DRAM1 messenger RNA (mRNA), ARSA mRNA, hsa-miR-2053 and lncRNA-RP1-86D1.3 were positive in 78.3%, 90%, 85%, and 83.3% of MPM patients, respectively. The RNA-based biomarker panel was able to discriminate between MPM patients and controls with high accuracy and their combined sensitivity reached 100% for the diagnosis of MPM. Kaplan-Meier analysis showed that hsa-miR-2053 is an independent prognostic factor of MPM. Conclusion Our preliminary data revealed that the chosen RNAs play an important role in driving MPM development and progression.

引用

页码：3203 / 3211

页数：9