Uptake of fluorescent nano beads into BY2-cells involves clathrin-dependent and clathrin-independent endocytosis

被引：189

作者：

Bandmann, Vera ^{[1
]}

Mueller, Jasmin Daniela ^{[1
]}

Koehler, Tim ^{[1
]}

Homann, Ulrike ^{[1
]}

机构：

[1] Tech Univ Darmstadt, D-64287 Darmstadt, Germany

来源：

FEBS LETTERS | 2012年 / 586卷 / 20期

关键词：

Fluorescent nano bead; Clathrin-independent endocytosis; Clathrin-dependent endocytosis; IKA; Wortmannin; BY-2; PATHWAYS;

D O I：

10.1016/j.febslet.2012.08.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To follow endocytosis in BY-2 cells we made use of fluorescent nano beads. Beads with 20 nm in diameter were internalised rapidly and accumulated partially in compartments also labelled by the endocytic marker FM4-64. Studies in BY-2 cells and protoplasts revealed that larger beads (100 nm) were excluded from uptake into turgescent and plasmolysed cells while protoplasts were able to internalise beads with a diameter of up to 1000 nm. Endocytosis of beads was only partially inhibited by the clathrin-specific inhibitor Ikarugamycin and strongly blocked by wortmannin. These results imply that uptake of beads involves clathrin-dependent and clathrin-independent endocytic mechanisms and supports the hypothesis that clathrin-independent endocytosis plays a general role in plants. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3626 / 3632

页数：7

共 17 条

[1] Clathrin-independent endocytosis contributes to uptake of glucose into BY-2 protoplasts [J].