Measurement of muscle disease by quantitative second-harmonic generation imaging

被引：73

作者：

Plotnikov, Sergey V. ^{[1
,2
]}

Kenny, Anne M. ^{[3
]}

Walsh, Stephen J. ^{[4
]}

Zubrowski, Beata ^{[3
]}

Joseph, Cherian ^{[3
]}

Scranton, Victoria L. ^{[1
]}

Kuchel, George A. ^{[3
]}

Dauser, Deborah ^{[4
]}

Xu, Manshan ^{[5
]}

Pilbeam, Carol C. ^{[5
]}

Adams, Douglas J. ^{[6
]}

Dougherty, Robert P. ^{[7
]}

Campagnola, Paul J. ^{[2
]}

Mohler, William A. ^{[1
,2
]}

机构：

[1] Univ Connecticut, Ctr Hlth, Dept Genet & Dev Biol, Farmington, CT 06030 USA

[2] Univ Connecticut, Ctr Hlth, Ctr Cell Anal & Modeling, Farmington, CT 06030 USA

[3] Univ Connecticut, Ctr Hlth, Ctr Aging, Farmington, CT 06030 USA

[4] Univ Connecticut, Ctr Hlth, Ctr Community Med & Hlth Care, Farmington, CT 06030 USA

[5] Univ Connecticut, Ctr Hlth, Dept Med, Farmington, CT 06030 USA

[6] Univ Connecticut, Ctr Hlth, Dept Orthoped Surg, Farmington, CT 06030 USA

[7] OptiNav Inc, Bellevue, WA 98004 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2008年 / 13卷 / 04期

基金：

美国国家科学基金会;

关键词：

second-harmonic generation; pattern recognition; scanning microscopy; lasers in medicine; laser application;

D O I：

10.1117/1.2967536

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Determining the health of muscle cells by in vivo imaging could impact the diagnosis and monitoring of a large number of congenital and acquired muscular or cardiac disorders. However, currently used technologies are hampered by insufficient resolution, lack of specificity, or invasiveness. We have combined intrinsic optical second-harmonic generation from sarcomeric myosin with a novel mathematical treatment of striation pattern analysis, to obtain measures of muscle contractile integrity that correlate strongly with the neuromuscular health of mice suffering from genetic, acquired, and age-related decline in skeletal muscle function. Analysis of biopsies from a pilot group of human volunteers suggests a similar power in quantifying sarcopenic changes in muscle integrity. These results provide the first strong evidence that quantitative image analysis of sarcomere pattern can be correlated with physiological function, and they invite the application of SHG imaging in clinical practice, either in biopsy samples or via microendoscopy. (C) 2008 Society of Photo-Optical Instrumentation Engineers.

引用

页数：11

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