Adenovirus-mediated small-interference RNA for in vivo silencing of angiotensin AT1a receptors in mouse brain

Because of the lack of pharmacological approaches, molecular genetic methods have been required to differentiate between angiotensin type 1( AT(1)) receptor subtypes AT(1a) and AT(1b). RNA interference is a new tool for the study of gene function, producing specific downregulation of protein expression. In this study, we used the small hairpin RNA (shRNA) cassette method to screen target sites for selectively silencing AT(1a) or AT(1b) receptor subtypes in cultured Neuro-2a cells using real-time RT-PCR. For in vivo functional studies, we used C57BL mice with arterial telemetric probes and computerized licking monitors to test the effect of adenovirus carrying the DNA sequence coding AT(1a) shRNA (Ad-AT(1a)-shRNA). Ad-AT(1a)-shRNA was injected into the lateral ventricle (intracerebroventricular) or the brain stem nucleus tractus solitaries/dorsal vagal nucleus (NTS/DVN) with measurement of water intake, blood pressure ( BP), and heart rate (HR) for up to 20 days after injection. Tissue culture studies verified the specificity and the efficiency of the constructs. In animal studies, beta-galactosidase staining and Ang receptor binding assays showed expression of shRNA and downregulation of Ang AT(1) receptors in the subfornical organ and NTS/DVN by > 70%. Intracerebroventricular injection of Ad-AT(1a)-shRNA increased water intake with no effect on BP or HR. In contrast, microinjection of Ad-AT(1a)-shRNA into NTS/DVN caused a decrease in BP with no effect on HR or water intake. Results demonstrate the use of the RNA interference method in site-directed silencing of gene expression and provide a method for the in vivo study of Ang AT(1) receptor function.