Quorum Sensing Inhibition in Pseudomonas aeruginosa PAO1 by Antagonistic Compound Phenylacetic Acid

被引：55
作者：
Musthafa, Khadar Syed [1 ]
Sivamaruthi, Bhagavathi Sundaram [1 ]
Pandian, Shunmugiah Karutha [1 ]
Ravi, Arumugam Veera [1 ]
机构：
[1] Alagappa Univ, Dept Biotechnol, Karaikkudi 630003, Tamil Nadu, India
来源：
CURRENT MICROBIOLOGY | 2012年 / 65卷 / 05期
关键词：
CAENORHABDITIS-ELEGANS; VIRULENCE FACTORS; IDENTIFICATION; COMMUNICATION; PLANTS;
D O I：
10.1007/s00284-012-0181-9
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
In Pseudomonas aeruginosa, quorum sensing (QS) autoinducer known as acyl homoserine lactone (AHL) acts as a key regulator in the expression of pathogenic characters. In this work, the efficiency of phenylacetic acid (PAA) in reducing the production of AHL-dependent factors in P. aeruginosa PAO1 was studied. PAA at a concentration of 200 mu g ml(-1) displayed significant reduction in QS-dependent pyocyanin, exopolysaccharide, and protease and elastase production in PAO1. In swimming inhibition assay, PAA-treated PAO1 cells exhibited poor motility in swimming agar plate. In in vivo analysis, PAO1-preinfected Caenorhabditis elegans showed enhanced survival when treated with PAA. PAA at the QS inhibitory concentration showed no growth inhibitory activity on PAO1. Results of the present study revealed the potential of PAA as antipathogenic compound to prevent QS-dependent pathogenicity of P. aeruginosa.
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收藏
页码：475 / 480
页数：6
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