Apoptotic and anti-adhesion effect of ajoene, a garlic derived compound, on the murine melanoma B16F10 cells:: possible role of caspase-3 and the α4β1 integrin

被引：31

作者：

Ledezma, E

Apitz-Castro, R

Cardier, J

机构：

[1] Inst Venezolano Invest Cient, Ctr Med Expt, Lab Patol Celular & Mol, Caracas 1020A, Venezuela

[2] Univ Oriente, Escuela Med, Ctr Invest Ciencias Salud, Puerto La Cruz, Venezuela

[3] Inst Venezolano Invest Cient, Ctr Biofis & Bioquim, Lab Trombosis Expt, Caracas 1020A, Venezuela

来源：

CANCER LETTERS | 2004年 / 206卷 / 01期

关键词：

ajoene; apoptosis; integrin; adhesion; melanoma; B16F10;

D O I：

10.1016/j.canlet.2003.10.031

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In this study we evaluated the hypothesis that the antitumor activity of ajoene could be associated with its apoptosis-inducing effect, and with its ability to block the expression of the alpha(4)beta(1) integrin, in the murine melanoma B 161710 cells. Ajoene induced a significant reduction in B16F10 viability (IC50 = 62 muM), in a dose-dependent manner. Flow cytometric analysis showed that the cytotoxic effect of this compound was associated with caspase-3 activation. Ajoene at 25 muM altered the alpha(4)beta(1) integrin expression on B16F10. and induced a significant reduction in the adhesion of these cells to an endothelial cell monolayer. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：35 / 41

页数：7

共 30 条

[1] EVIDENCE FOR DIRECT COUPLING OF PRIMARY AGONIST RECEPTOR INTERACTION TO THE EXPOSURE OF FUNCTIONAL IIB-IIIA COMPLEXES IN HUMAN BLOOD-PLATELETS - RESULTS FROM STUDIES WITH THE ANTIPLATELET COMPOUND AJOENE [J].