Phenolic components and antioxidant activity of Fernblock®, an aqueous extract of the aerial parts of the fern Polypodium leucotomos

被引:50
作者
García, F
Pivel, JP
Guerrero, A
Brieva, A
Martínez-Alcázar, M
Caamaño-Somoza, M
González, S
机构
[1] Ind Farmaceut Cantabria, Madrid 28043, Spain
[2] Univ San Pablo, Fac Farm, Serv Espectrometria Masas, Madrid, Spain
[3] Mem Sloan Kettering Canc Ctr, Dermatol Serv, New York, NY 10021 USA
来源
METHODS AND FINDINGS IN EXPERIMENTAL AND CLINICAL PHARMACOLOGY | 2006年 / 28卷 / 03期
关键词
antioxidant; FRAP; HPLC-electrochemical detection; HPLC-mass spectrum; phenolics; polypodium;
D O I
10.1358/mf.2006.28.3.985227
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Fernblock (R), an aqueous extract of the aerial parts of the fern Polypodium leucotomos, used as raw, material for topical and oral photoprotective formulations, was fractioned by HPLC and the main components with antioxidant capability were identified by means of UV spectra, electrochemical detection, and MS". Phenolic compounds were identified as 3,4-dihydroxyhenzoic acid, 4-hydroxybenzoic acid, vanillic acid, caffeic acid, 4-hydroxycinnamic acid, 4-hydrosycinnamoyl-quinic acid, ferulic acid, and five chlorogenic acid isomers. Total firric antioxidant capacity (FRAP) of HPLC eluted fractions was measured. The results suggest that the herein identified compounds support, at least partially, the antioxidant and radical scavenging capacities of Fernblock. (c) 2006 Prous Science. All rights reserved.
引用
收藏
页码:157 / 160
页数:4
相关论文
共 30 条
[1]   Analysis of flavonoids and other phenolic compounds using high-performance liquid chromatography with coulometric array detection: Relationship to antioxidant activity [J].
Aaby, K ;
Hvattum, E ;
Skrede, G .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2004, 52 (15) :4595-4603
[2]   The photodimerisation of trans-cinnamic acid and its derivatives:: a study by vibrational microspectroscopy [J].
Allen, SDM ;
Almond, MJ ;
Bruneel, JL ;
Gilbert, A ;
Hollins, P ;
Mascetti, J .
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2000, 56 (12) :2423-2430
[3]   THE SHIKIMATE PATHWAY - A METABOLIC TREE WITH MANY BRANCHES [J].
BENTLEY, R .
CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1990, 25 (05) :307-384
[4]  
Buettner GR, 2000, TERATOLOGY, V62, P234, DOI 10.1002/1096-9926(200010)62:4<234::AID-TERA10>3.0.CO
[5]  
2-9
[6]   Polypodium leucotomos extract inhibits trans-urocanic acid photoisomerization and photodecomposition [J].
Capote, R ;
Alonso-Lebrero, JL ;
García, F ;
Brieva, A ;
Pivel, JP ;
González, S .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2006, 82 (03) :173-179
[7]   Hierarchical scheme for LC-MSn identification of chlorogenic acids [J].
Clifford, MN ;
Johnston, KL ;
Knight, S ;
Kuhnert, N .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2003, 51 (10) :2900-2911
[8]   Ultraviolet radiation screening compounds [J].
Cockell, CS ;
Knowland, J .
BIOLOGICAL REVIEWS, 1999, 74 (03) :311-345
[9]  
DUKE JA, CHEM THEIR BIOL ACTI
[10]   LC/MS/MS characterization of phenolic constituents in dried plums [J].
Fang, NB ;
Yu, SG ;
Prior, RL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (12) :3579-3585