Species identification and molecular characterization of Cronobacter spp. isolated from food imported over nine years into Beijing, China

被引：11

作者：

Wang, Qi ^{[1
]}

Forsythe, Stephen James ^{[2
]}

Zhao, Xiao-Juan ^{[1
]}

Wang, Zi-Wei ^{[1
]}

Li, Dan ^{[1
]}

Ma, Dan ^{[1
]}

Cao, Jia-Yue ^{[1
]}

Zeng, Jing ^{[1
]}

机构：

[1] Beijing Customs, Tianshuiyuan St 6, Beijing 100026, Peoples R China

[2] Foodmicrobe Com, Adams Hill, Keyworth NG12 5GY, Notts, England

来源：

FOOD MICROBIOLOGY | 2019年 / 82卷

关键词：

Cronobacter; MALDI-TOF MS; MLST; PCR-serotyping; Species identification; ASSISTED LASER-DESORPTION; MALDI-TOF MS; FLIGHT MASS-SPECTROMETRY; ANTIGEN GENE CLUSTERS; IONIZATION-TIME; MICROBIOLOGICAL IDENTIFICATION; ENTEROBACTER-SAKAZAKII; DIFFERENTIATION; DIVERSITY;

D O I：

10.1016/j.fm.2019.01.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cronobacter spp. are associated with serious infections in neonates with the clinical presentations of necrotizing enterocolitis, bacteraemia and meningitis. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to identify 203 Cronobacter isolates from imported food during 2006-2015 with an optimized in-house database. The isolates were predominantly C. sakazakii (88.18%), followed by C. malonaticus (8.37%), C. muytjensii (1.48%), C. turicensis (0.99%) and C. dublinensis (0.99%). The result was totally consistent with that of fusA allele sequencing. 12.32% (25/203) of isolates gave inconsistent spectra following separate protein extractions. Sixty C. sakazakii isolates and 24 isolates from the other four species were chosen for multi-locus sequence type analyses (MLST) and PCR-serotyping. Thirty-one sequence types were identified. The common sequence types were ST1 (19/60) and ST4 (13/60) for C. sakazakii and ST7 (12/17) for C. malonaticus. The primary serotypes were Csak O:1 (30/60), Csak O:2 (25/60) and Cmal O:2 (16/17) for C. sakazakii and C. malonaticus isolates, respectively. In conclusion, appropriate in-house database could make MALDI-TOF MS method identifying Cronobacter spp. isolates to the species level. But the spectra data were not sufficiently consistent for subtyping, unlike MLST. The Cronobacter spp. isolates have a high diversity including recognized pathovars.

引用

页码：11 / 19

页数：9

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