Structure and immunology of a heteroglycan from somatic hybrid mushroom of Pleurotus florida and Calocybe indica var. APK2

被引：16

作者：

Dey, Biswajit ^{[1
]}

Bhunia, Sanjoy K. ^{[1
]}

Maity, Kankan K. ^{[1
]}

Patra, Sukesh ^{[1
]}

Mandal, Soumitra ^{[1
]}

Behera, Birendra ^{[2
]}

Maiti, Tapas K. ^{[2
]}

Sikdar, Samir R. ^{[3
]}

Islam, Syed S. ^{[1
]}

机构：

[1] Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India

[2] Indian Inst Technol IIT Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India

[3] Bose Inst, Div Plant Biol, Kolkata 700054, W Bengal, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 52卷

关键词：

Somatic hybrid PCH3FB; Pleurotus florida; Calocybe indica var. APK2; Mushroom polysaccharide; NMR spectroscopy; Immunoactivation; EDIBLE MUSHROOM; CHEMICAL-ANALYSIS; ALKALINE EXTRACT; POLYSACCHARIDES; GLUCAN; (1->3)-BETA-D-GLUCAN; MACROPHAGES; ANTITUMOR; FRACTION; BODIES;

D O I：

10.1016/j.ijbiomac.2012.09.028

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A water soluble polysaccharide was isolated from the aqueous extract of the fruit bodies of somatic hybrid mushroom PCH3FB, obtained through protoplast fusion between the strains Pleurotus florida and Calocybe indica var. APK2. On the basis of sugar analysis, methylation analysis, periodate oxidation, and NMR studies (H-1, C-13, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC and HMBC), the structure of the repeating unit of the polysaccharide was established as: [GRAPHICS] This molecule showed macrophage, splenocyte and thymocyte activations. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：312 / 318

页数：7

共 35 条

[1]

ADAMS DO, 1984, ANNU REV IMMUNOL, V2, P283, DOI 10.1146/annurev.iy.02.040184.001435

[2]

Adenipekun C., 2006, Pakistan Journal of Nutriotion, V5, P597, DOI DOI 10.3923/PJN.2006.597.600

[3] NMR-SPECTROSCOPY IN THE STRUCTURAL ELUCIDATION OF OLIGOSACCHARIDES AND GLYCOSIDES [J].

AGRAWAL, PK .

PHYTOCHEMISTRY, 1992, 31 (10) :3307-3330

[4] Glechoma hederacea inhibits inflammatory mediator release in IFN-γ and LPS-stimulated mouse peritoneal macrophages [J].

An, Hyo-Jin ;

Jeong, Hyun-Ja ;

Um, Jae-Young ;

Kim, Hyung-Min ;

Hong, Seung-Heon .

JOURNAL OF ETHNOPHARMACOLOGY, 2006, 106 (03) :418-424

[5] Intergeneric protoplast fusion between Calocybe indica (milky mushroom) and Pleurotus florida aids in the qualitative and quantitative improvement of sporophore of the milky mushroom [J].

Chakraborty, Uttara ;

Sikdar, Samir Ranjan .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2010, 26 (02) :213-225

[6] A SIMPLE AND RAPID METHOD FOR THE PERMETHYLATION OF CARBOHYDRATES [J].

CIUCANU, I ;

KEREK, F .

CARBOHYDRATE RESEARCH, 1984, 131 (02) :209-217

[7] Glucans of Pleurotus florida blue variant: Isolation, purification, characterization and immunological studies [J].

Dey, Biswajit ;

Bhunia, Sanjoy K. ;

Maity, Kankan K. ;

Patra, Sukesh ;

Mandal, Soumitra ;

Maiti, Swatilekha ;

Maiti, Tapas K. ;

Sikdar, Samir R. ;

Islam, Syed S. .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2012, 50 (03) :591-597

[8] Chemical analysis of an immunoenhancing water-soluble polysaccharide of an edible mushroom, Pleurotus florida blue variant [J].

Dey, Biswajit ;

Bhunia, Sanjoy K. ;

Maity, Kankan K. ;

Patra, Sukesh ;

Mandal, Soumitra ;

Maiti, Swatilekha ;

Maiti, Tapas K. ;

Sikdar, Samir R. ;

Islam, Syed S. .

CARBOHYDRATE RESEARCH, 2010, 345 (18) :2736-2741

[9]

Duenas Chaso M. T., 1997, CARBOHYD RES, V303, P453

[10] DETERMINATION OF D AND L CONFIGURATION OF NEUTRAL MONOSACCHARIDES BY HIGH-RESOLUTION CAPILLARY GLC [J].

GERWIG, GJ ;

KAMERLING, JP ;

VLIEGENTHART, JFG .

CARBOHYDRATE RESEARCH, 1978, 62 (02) :349-357

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