Optogenetic reporters

被引：31

作者：

Alford, Spencer C.

Wu, Jiahui

Zhao, Yongxin

Campbell, Robert E.

Knoepfel, Thomas ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

来源：

BIOLOGY OF THE CELL | 2013年 / 105卷 / 01期

基金：

美国国家卫生研究院;

关键词：

Cellular imaging; Fluorescence techniques; Fluorescent proteins; Genetically encoded indicators; Protein domains; GREEN FLUORESCENT PROTEIN; RESONANCE ENERGY-TRANSFER; TRANSFER-BASED BIOSENSORS; LIVING CELLS; IN-VIVO; CA2+ INDICATORS; VOLTAGE SENSOR; TRANSFER BRET; PHYSIOLOGICAL CONDITIONS; COMPLEMENTATION ANALYSIS;

D O I：

10.1111/boc.201200054

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The discovery of naturally evolved fluorescent proteins and their subsequent tuning by protein engineering provided the basis for a large family of genetically encoded biosensors that report a variety of physicochemical processes occurring in living tissue. These optogenetic reporters are powerful tools for live-cell microscopy and quantitative analysis at the subcellular level. In this review, we present an overview of the transduction mechanisms that have been exploited for engineering these genetically encoded reporters. Finally, we discuss current and future efforts towards the combined use of various optogenetic actuators and reporters for simultaneously controlling and imaging the physiology of cells and tissues.

引用

收藏

页码：14 / 29

页数：16

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