Molecularly imprinted polymer of 5-methyluridine for solid-phase extraction of pyrimidine nucleoside cancer markers in urine

被引：47

作者：

Jegourel, Damien ^{[1
]}

Delepee, Raphael ^{[1
]}

Breton, Florent ^{[1
]}

Rolland, Antoine ^{[2
]}

Vidal, Richard ^{[2
]}

Agrofoglio, Luigi A. ^{[1
]}

机构：

[1] Univ Orleans, CNRS, ICOA, UMR 6005, F-45067 Orleans 2, France

[2] ESTAPOR Merck Chim, F-45800 Pithiviers, France

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2008年 / 16卷 / 19期

关键词：

5-methyluridine; cancer markers; molecularly imprinting polymer; pyrimidinic nucleosides; MISPE; urine;

D O I：

10.1016/j.bmc.2008.08.063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Normal and modified urinary nucleosides represent potential biomarkers for cancer diagnosis. To selectively extract modified nucleosides, we developed a molecularly imprinted polymer (MIP) of 5-methyluridine as selective material for molecularly imprinted solid-phase extraction (MISPE). The MIPs were obtained from vinyl-phenylboronate ester derivative of the template, acrylamide and pentaerythritol triacrylate co-polymer, and were tested in batch and cartridge experiments with aqueous samples. Our results indicated that the imprinted polymer was selective for pyrimidine nucleosides with a K(d) and a B(max) of 46 mu M and 18 mu mol/g, respectively. Finally, a MISPE of the most common pyrimidine nucleoside cancer markers in urine sample was realized. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：8932 / 8939

页数：8

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