Molecularly imprinted polymer of 5-methyluridine for solid-phase extraction of pyrimidine nucleoside cancer markers in urine

被引：44

作者：

Jegourel, Damien ^{[1
]}

Delepee, Raphael ^{[1
]}

Breton, Florent ^{[1
]}

Rolland, Antoine ^{[2
]}

Vidal, Richard ^{[2
]}

Agrofoglio, Luigi A. ^{[1
]}

机构：

[1] Univ Orleans, CNRS, ICOA, UMR 6005, F-45067 Orleans 2, France

[2] ESTAPOR Merck Chim, F-45800 Pithiviers, France

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2008年 / 16卷 / 19期

关键词：

5-methyluridine; cancer markers; molecularly imprinting polymer; pyrimidinic nucleosides; MISPE; urine;

D O I：

10.1016/j.bmc.2008.08.063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Normal and modified urinary nucleosides represent potential biomarkers for cancer diagnosis. To selectively extract modified nucleosides, we developed a molecularly imprinted polymer (MIP) of 5-methyluridine as selective material for molecularly imprinted solid-phase extraction (MISPE). The MIPs were obtained from vinyl-phenylboronate ester derivative of the template, acrylamide and pentaerythritol triacrylate co-polymer, and were tested in batch and cartridge experiments with aqueous samples. Our results indicated that the imprinted polymer was selective for pyrimidine nucleosides with a K(d) and a B(max) of 46 mu M and 18 mu mol/g, respectively. Finally, a MISPE of the most common pyrimidine nucleoside cancer markers in urine sample was realized. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：8932 / 8939

页数：8

共 60 条

[1] An NMR, IR and theoretical investigation of 1H Chemical Shifts and hydrogen bonding in phenols
Abraham, Raymond J.
Mobli, Mehdi
[J]. MAGNETIC RESONANCE IN CHEMISTRY, 2007, 45 (10) : 865 - 877
[2] Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003
Alexander, C
Andersson, HS
Andersson, LI
Ansell, RJ
Kirsch, N
Nicholls, IA
O'Mahony, J
Whitcombe, MJ
[J]. JOURNAL OF MOLECULAR RECOGNITION, 2006, 19 (02) : 106 - 180
[3] Separation of individual antiviral nucleotide prodrugs from synthetic mixtures using cross-reactivity of a molecularly imprinted stationary phase
Allender, CJ
Brain, KR
Ballatore, C
Cahard, D
Siddiqui, A
McGuigan, C
[J]. ANALYTICA CHIMICA ACTA, 2001, 435 (01) : 107 - 113
[4] ARSHADY R, 1981, MACROMOL CHEM PHYS, V182, P687
[5] Hydrogen-bonded complexes of diaminopyridines and diaminotriazines: Opposite effect of acylation on complex stabilities
Beijer, FH
Sijbesma, RP
Vekemans, JAJM
Meijer, EW
Kooijman, H
Spek, AL
[J]. JOURNAL OF ORGANIC CHEMISTRY, 1996, 61 (18) : 6371 - 6380
[6] Beijer FH, 1998, ANGEW CHEM INT EDIT, V37, P75, DOI 10.1002/(SICI)1521-3773(19980202)37:1/2<75::AID-ANIE75>3.0.CO
[7] 2-R
[8] Analysis and validation of the phosphorylated metabolites of two anti-human immunodeficiency virus nucleotides (stavudine and didanosine) by pressure-assisted CE-ESI-MS/MS in cell extracts: Sensitivity enhancement by the use of perfluorinated acids and alcohols as coaxial sheath-liquid make-up constituents
Bezy, Vincent
Chaimbault, Patrick
Morin, Philippe
Unger, Steve E.
Bernard, Marie-Charlotte
Agrofoglio, Luigi A.
[J]. ELECTROPHORESIS, 2006, 27 (12) : 2464 - 2476
[9] Binary and ternary phenylboronic acid complexes with saccharides and Lewis bases
Bosch, LI
Fyles, TM
James, TD
[J]. TETRAHEDRON, 2004, 60 (49) : 11175 - 11190
[10] The dissociation constants of the chlorophenyl and phenetyl boric acid
Branch, GEK
Yabroff, DL
Bettman, B
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1934, 56 : 937 - 941

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