Macular vascular density at the superficial capillary plexus using the optical coherence tomography angiography

Purpose: The aim of this study was to assess the effects of age, gender, axial length and retinal thickness on the vascular density in a normal population using the Optovue optical coherence tomography angiography (OCT-A). Methods: A total of 209 eyes of 209 healthy subjects were scanned using the OCT-A on an outpatient basis. Patients were 20-75 years of age. The built-in software covered a 3x3 mm circle centered at the macula. The circle was divided into five sectors: a 1 mm central foveal circle and an upper, lower, nasal and temporal sector each spanning 1-3 mm away from the central circle. The vascular density (VD) at the superficial capillary plexus was measured, which spanned from the inner limiting membrane to the inner plexiform layer. Results: The fovea was the least dense area (32.5%+/- 5.9%), and the temporal area was the densest (52.4%+/- 4.4%). Similarly, the fovea was the thinnest part of the retina (237.8 +/- 20.7 mu m); however, the nasal sector was the thickest (308.5 +/- 15.1 mu m). We found a correlation of the retinal thickness (r=0.541, P<0.001) with VD only at the fovea. Similarly, males had a greater density at the fovea (P=0.002). All regions significantly and negatively correlated with age even after adjusting for axial length. The age sees a decline in the density after the fifth decade where the variability also seems to increase. Conclusion: This study provides normative data for the Pakistani population. Additionally, it demonstrates that VD is affected by the retinal thickness at the fovea and the density begins to decline after the fifth decade.