siRNA Interference with a Proliferation-Inducing Ligand Gene in the Sgr-7901 Gastric Carcinoma Cell Line

被引：4
作者：
Ni, Shao-Zhong [1 ,2 ]
Cao, Hong-Yong [2 ]
Chen, Zheng [1 ]
Zhu, Yi [1 ,3 ]
Xu, Ze-Kuan [1 ,3 ]
机构：
[1] Nanjing Med Univ, Dept Gen Surg, Affiliated Hosp 1, Nanjing, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Dept Gen Surg, Affiliated Nanjing Hosp 1, Nanjing, Jiangsu, Peoples R China
[3] Jiangsu Prov Acad Clin Med, Inst Tumor Biol, Nanjing, Jiangsu, Peoples R China
来源：
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION | 2012年 / 13卷 / 04期
关键词：
Proliferation-inducing ligand (APRIL) gene; RNA interference; gastric carcinoma; tyrosine kinase; NECROSIS-FACTOR FAMILY; B-CELL; CANCER CELLS; APRIL; EXPRESSION; GROWTH; ADENOCARCINOMA; PROGRESSION; APOPTOSIS; SURVIVAL;
D O I：
10.7314/APJCP.2012.13.4.1511
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Objective: The present study aimed to investigate the influence of siRNA interference with a proliferation-inducing ligand (APRIL) gene on gastric carcinoma sgr-7901 cell apoptosis. Correlations between APRIL silencing and tyrosine kinase (trka) expression were also explored. Methods: Two APRIL-silencing siRNA vectors were constructed, and transfected into human gastric carcinoma sgr-7901 cells, expression before and after transfection being detected using RT-PCR and western blot analyses. The expression of 15 trka genes was detected using RT-PCR and apoptotic rates of sgr-7901 were assessed by flow cytometry. Results: The expression levels of receptor trka genes were significantly decreased, and the apoptotic rate of sgr-7901 was significantly increased after transfection (P < 0.05). Conclusion: APRIL gene silencing can increase the apoptotic rate of gastric carcinoma cells, and inhibit the expression of receptor trka genes. There is a correlation between the signaling pathways of APRIL and trka.
引用
收藏
页码：1511 / 1514
页数：4
相关论文
共 29 条
  • [1] Signalling pathways of the TNF superfamily: A double-edged sword
    Aggarwal, BB
    [J]. NATURE REVIEWS IMMUNOLOGY, 2003, 3 (09) : 745 - 756
  • [2] HER2-mediated upregulation of MMP-1 is involved in gastric cancer cell invasion
    Bao, Wei
    Fu, Hai-Jing
    Jia, Lin-Tao
    Zhang, Yong
    Li, Wei
    Jin, Bo-Quan
    Yao, Li-Bo
    Chen, Si-Yi
    Yang, An-Gang
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2010, 499 (1-2) : 49 - 55
  • [3] Impaired IgA class switching in APRIL-deficient mice
    Castigli, E
    Scott, S
    Dedeoglu, F
    Bryce, P
    Jabara, H
    Bhan, AK
    Mizoguchi, E
    Geha, RS
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (11) : 3903 - 3908
  • [4] Cho-Chung YS, 1999, CURR OPIN MOL THER, V1, P386
  • [5] Antisense DNAs as targeted genetic medicine to treat cancer
    Cho-Chung, YS
    [J]. ARCHIVES OF PHARMACAL RESEARCH, 2003, 26 (03) : 183 - 191
  • [6] Perioperative chemotherapy versus surgery alone for resectable gastroesophageal cancer
    Cunningham, David
    Allum, William H.
    Stenning, Sally P.
    Thompson, Jeremy N.
    Van de Velde, Cornelis J. H.
    Nicolson, Marianne
    Scarffe, J. Howard
    Lofts, Fiona J.
    Falk, Stephen J.
    Iveson, Timothy J.
    Smith, David B.
    Langley, Ruth E.
    Verma, Monica
    Weeden, Simon
    Chua, Yu Jo
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2006, 355 (01) : 11 - 20
  • [7] APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth
    Hahne, M
    Kataoka, T
    Schröter, M
    Hofmann, K
    Irmler, M
    Bodmer, JL
    Schneider, P
    Bornard, T
    Holler, N
    French, LE
    Sordat, B
    Rimoldi, D
    Tschopp, J
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 188 (06) : 1185 - 1190
  • [8] Gastric cancer
    Hohenberger, P
    Gretschel, S
    [J]. LANCET, 2003, 362 (9380) : 305 - 315
  • [9] Huang WL, 2005, SIGNAL TRANSDUCTION, P151
  • [10] Cancer statistics, 2006
    Jemal, A
    Siegel, R
    Ward, E
    Murray, T
    Xu, JQ
    Smigal, C
    Thun, MJ
    [J]. CA-A CANCER JOURNAL FOR CLINICIANS, 2006, 56 (02) : 106 - 130
123