Stem cell characteristics of human trabecular bone-derived cells

被引：157

作者：

Sottile, V ^{[1
]}

Halleux, C ^{[1
]}

Bassilana, F ^{[1
]}

Keller, H ^{[1
]}

Seuwen, K ^{[1
]}

机构：

[1] Novartis Pharma AG, Res, CH-4002 Basel, Switzerland

来源：

BONE | 2002年 / 30卷 / 05期

关键词：

cell differentiation; osteoblast; adipocyte chondrocyte; progenitor cell; limiting dilution;

D O I：

10.1016/S8756-3282(02)00674-9

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Human trabecular bone-derived cells (HTBs) have been used for many years as osteoblast progenitors. In this study we tested whether HTBs have stem cell characteristics; that is, whether they are pluripotent and able to self-renew. We show that HTBs readily differentiate into osteoblasts, chondrocytes, and adipocytes if subjected to the appropriate differentiating conditions. Importantly, differentiation into these three lineages is maintained in single cell clones derived by limiting dilution, following expansion over more than 20 cumulative population doublings. We conclude that cultures of HTBs are equivalent to cultures of ''mesenchymal stem cells'' (MSCs) isolated from bone marrow. (C) 2002 by Elsevier Science Inc. All rights reserved.

引用

页码：699 / 704

页数：6

共 24 条

[1] PRODUCTION OF OSTEOCALCIN BY HUMAN-BONE CELLS-INVITRO - EFFECTS OF 1,25(OH)2D3, 24,25(OH)2D3, PARATHYROID-HORMONE, AND GLUCOCORTICOIDS [J].

BERESFORD, JN ;

GALLAGHER, JA ;

POSER, JW ;

RUSSELL, RGG .

METABOLIC BONE DISEASE & RELATED RESEARCH, 1984, 5 (05) :229-234

[2] MESENCHYMAL STEM-CELLS [J].

CAPLAN, AI .

JOURNAL OF ORTHOPAEDIC RESEARCH, 1991, 9 (05) :641-650

[3]

Friedenstein A J, 1980, Haematol Blood Transfus, V25, P19

[4] DEVELOPMENT OF FIBROBLAST COLONIES IN MONOLAYER CULTURES OF GUINEA-PIG BONE MARROW AND SPLEEN CELLS [J].

FRIEDENSTEIN, AJ ;

CHAILAKHJAN, RK ;

LALYKINA, KS .

CELL AND TISSUE KINETICS, 1970, 3 (04) :393-+

[5] PRECURSOR CELLS OF MECHANOCYTES [J].

FRIEDENSTEIN, AJ .

INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1976, 47 :327-359

[6] HETEROTOPIC TRANSPLANTS OF BONE MARROW - ANALYSIS OF PRECURSOR CELLS FOR OSTEOGENIC AND HEMATOPOIETIC TISSUES [J].

FRIEDENSTEIN, AJ ;

PETRAKOVA, KV ;

KUROLESOVA, AI ;

FROLOVA, GP .

TRANSPLANTATION, 1968, 6 (02) :230-+

[7]

Gimble JM, 1996, MOL PHARMACOL, V50, P1087

[8]

Halleux C., 2001, Journal of Musculoskeletal & Neuronal Interactions, V2, P71

[9]

Jaiswal N, 1997, J CELL BIOCHEM, V64, P295, DOI 10.1002/(SICI)1097-4644(199702)64:2<295::AID-JCB12>3.3.CO

[10]

2-6

← 1 2 3 →