Human Pluripotent Stem Cell Culture: Considerations for Maintenance, Expansion, and Therapeutics

被引：261

作者：

Chen, Kevin G. ^{[1
]}

Mallon, Barbara S. ^{[1
]}

McKay, Ronald D. G. ^{[2
]}

Robey, Pamela G. ^{[3
]}

机构：

[1] Natl Inst Neurol Disorders & Stroke, NIH Stem Cell Unit, NIH, Bethesda, MD 20892 USA

[2] Lieber Inst Brain Dev, Baltimore, MD 21205 USA

[3] Natl Inst Dent & Craniofacial Res, Craniofacial & Skeletal Dis Branch, NIH, Bethesda, MD 20892 USA

来源：

CELL STEM CELL | 2014年 / 14卷 / 01期

基金：

美国国家卫生研究院;

关键词：

TERM SELF-RENEWAL; SYNTHETIC SURFACES; SUSPENSION-CULTURE; DIFFERENTIATION; GROWTH; DERIVATION; LINES; PROPAGATION; GENERATION; INDUCTION;

D O I：

10.1016/j.stem.2013.12.005

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Human pluripotent stem cells (hPSCs) provide powerful resources for application in regenerative medicine and pharmaceutical development. In the past decade, various methods have been developed for large-scale hPSC culture that rely on combined use of multiple growth components, including media containing various growth factors, extracellular matrices, 3D environmental cues, and modes of multicellular association. In this Protocol Review, we dissect these growth components by comparing cell culture methods and identifying the benefits and pitfalls associated with each one. We further provide criteria, considerations, and suggestions to achieve optimal cell growth for hPSC expansion, differentiation, and use in future therapeutic applications.

引用

页码：13 / 26

页数：14

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