ITS2 Database V: Twice as Much

被引:214
作者
Ankenbrand, Markus J. [1 ]
Keller, Alexander [1 ]
Wolf, Matthias [2 ]
Schultz, Joerg [2 ]
Foerster, Frank [2 ]
机构
[1] Julius Maximilian Univ, Dept Anim Ecol & Trop Biol, Wurzburg, Germany
[2] Julius Maximilian Univ, Dept Bioinformat, Wurzburg, Germany
关键词
barcoding; database; internal transcribed spacer; phylogeny; sequence-structure; toolbox; INTERNAL TRANSCRIBED SPACER; DNA BARCODE; RNA; SEQUENCES; REGION;
D O I
10.1093/molbev/msv174
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The internal transcribed spacer 2 (ITS2) is a well-established marker for phylogenetic analyses in eukaryotes. A reliable resource for reference sequences and their secondary structures is the ITS2 database (http://its2.bioapps.biozentrum.uniwuerzburg.de/). However, the database was last updated in 2011. Here, we present a major update of the underlying data almost doubling the number of entities. This increases the number of taxa represented within all major eukaryotic clades. Moreover, additional data has been added to underrepresented groups and some new groups have been added. The broader coverage across the tree of life improves phylogenetic analyses and the capability of ITS2 as a DNA barcode.
引用
收藏
页码:3030 / 3032
页数:3
相关论文
共 27 条
  • [1] Acland A, 2013, NUCLEIC ACIDS RES, V41, pD8, DOI [10.1093/nar/gks1189, 10.1093/nar/gkx1095, 10.1093/nar/gkq1172]
  • [2] The Revised Classification of Eukaryotes
    Adl, Sina M.
    Simpson, Alastair G. B.
    Lane, Christopher E.
    Lukes, Julius
    Bass, David
    Bowser, Samuel S.
    Brown, Matthew W.
    Burki, Fabien
    Dunthorn, Micah
    Hampl, Vladimir
    Heiss, Aaron
    Hoppenrath, Mona
    Lara, Enrique
    le Gall, Line
    Lynn, Denis H.
    McManus, Hilary
    Mitchell, Edward A. D.
    Mozley-Stanridge, Sharon E.
    Parfrey, Laura W.
    Pawlowski, Jan
    Rueckert, Sonja
    Shadwick, Laura
    Schoch, Conrad L.
    Smirnov, Alexey
    Spiegel, Frederick W.
    [J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY, 2012, 59 (05) : 429 - 493
  • [3] Phylogenetic Utility of the Internal Transcribed Spacers of Nuclear Ribosomal DNA in Plants: An Example from the Compositae
    Baldwin, Bruce G.
    [J]. MOLECULAR PHYLOGENETICS AND EVOLUTION, 1992, 1 (01) : 3 - 16
  • [4] Chen SL, 2010, PLOS ONE, V5, DOI [10.1371/journal.pone.0015633, 10.1371/journal.pone.0008613]
  • [5] Is there a molecular key to the level of "biological species" in eukaryotes? A DNA guide
    Coleman, Annette W.
    [J]. MOLECULAR PHYLOGENETICS AND EVOLUTION, 2009, 50 (01) : 197 - 203
  • [6] ITS2 is a double-edged tool for eukaryote evolutionary comparisons
    Coleman, AW
    [J]. TRENDS IN GENETICS, 2003, 19 (07) : 370 - 375
  • [7] The NCBI Taxonomy database
    Federhen, Scott
    [J]. NUCLEIC ACIDS RESEARCH, 2012, 40 (D1) : D136 - D143
  • [8] Evaluating multiplexed next-generation sequencing as a method in palynology for mixed pollen samples
    Keller, A.
    Danner, N.
    Grimmer, G.
    Ankenbrand, M.
    von der Ohe, K.
    von der Ohe, W.
    Rost, S.
    Haertel, S.
    Steffan-Dewenter, I.
    [J]. PLANT BIOLOGY, 2015, 17 (02) : 558 - 566
  • [9] 5.8S-28S rRNA interaction and HMM-based ITS2 annotation
    Keller, Alexander
    Schleicher, Tina
    Schultz, Joerg
    Mueller, Tobias
    Dandekar, Thomas
    Wolf, Matthias
    [J]. GENE, 2009, 430 (1-2) : 50 - 57
  • [10] Including RNA secondary structures improves accuracy and robustness in reconstruction of phylogenetic trees
    Keller, Alexander
    Foerster, Frank
    Mueller, Tobias
    Dandekar, Thomas
    Schultz, Joerg
    Wolf, Matthias
    [J]. BIOLOGY DIRECT, 2010, 5