Spatial Dynamics of Chromosome Translocations in Living Cells

被引：238

作者：

Roukos, Vassilis ^{[1
]}

Voss, Ty C. ^{[2
]}

Schmidt, Christine K. ^{[1
]}

Lee, Seungtaek ^{[3
]}

Wangsa, Darawalee ^{[4
]}

Misteli, Tom ^{[1
]}

机构：

[1] NCI, Bethesda, MD 20892 USA

[2] NCI, High Throughput Imaging Facil, Bethesda, MD 20892 USA

[3] PerkinElmer Hlth Sci Inc, Waltham, MA 02451 USA

[4] NCI, Genet Branch, Bethesda, MD 20892 USA

来源：

SCIENCE | 2013年 / 341卷 / 6146期

基金：

美国国家卫生研究院;

关键词：

DOUBLE-STRAND BREAKS; DNA-PKCS; MOBILITY; RECOMBINATION; REPAIR;

D O I：

10.1126/science.1237150

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chromosome translocations are a hallmark of cancer cells. We have developed an experimental system to visualize the formation of translocations in living cells and apply it to characterize the spatial and dynamic properties of translocation formation. We demonstrate that translocations form within hours of the occurrence of double-strand breaks (DSBs) and that their formation is cell cycle-independent. Translocations form preferentially between prepositioned genome elements, and perturbation of key factors of the DNA repair machinery uncouples DSB pairing from translocation formation. These observations generate a spatiotemporal framework for the formation of translocations in living cells.

引用

收藏

页码：660 / 664

页数：5

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